Detailed information of TA system TA020555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 3138465..3139013
• Replicon: chromosome
• Accession: NC_002945
• Organism: Mycobacterium bovis AF2122/97

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TFG5
• Locus tag: BQ2027_RS14745
• Protein ID: WP_003414602.1
• Coordinates: 3138750..3139013 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: O33347
• Locus tag: BQ2027_RS14740
• Protein ID: WP_003414599.1
• Coordinates: 3138465..3138746 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BQ2027_RS14715 (BQ2027_MB2886C)	3134088..3134945	-	858	WP_003899513.1	type I methionyl aminopeptidase	-
BQ2027_RS14720 (BQ2027_MB2887C)	3134987..3135571	-	585	WP_010950792.1	DUF1707 domain-containing protein	-
BQ2027_RS14725 (BQ2027_MB2887A)	3135675..3135923	+	249	WP_003913411.1	antitoxin VapB23	-
BQ2027_RS14730 (BQ2027_MB2888)	3135920..3136300	+	381	WP_003414592.1	type II toxin-antitoxin system VapC family toxin	-
BQ2027_RS14735 (BQ2027_MB2889C)	3136382..3138193	-	1812	WP_003414596.1	penicillin-binding protein	-
BQ2027_RS14740 (BQ2027_MB2890)	3138465..3138746	+	282	WP_003414599.1	type II toxin-antitoxin system antitoxin RelF	Antitoxin
BQ2027_RS14745 (BQ2027_MB2891)	3138750..3139013	+	264	WP_003414602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BQ2027_RS14750 (BQ2027_MB2892C)	3139386..3140240	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
BQ2027_RS14755 (BQ2027_MB2893C)	3140296..3141459	-	1164	WP_010950793.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
BQ2027_RS14760 (BQ2027_MB2894C)	3141476..3142690	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
BQ2027_RS14765 (BQ2027_MB2895C)	3142698..3143939	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10204.82 Da
• Isoelectric Point: 11.5078

>T20555 WP_003414602.1 NC_002945:3138750-3139013 [Mycobacterium tuberculosis variant bovis AF2122/97]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Nucleotide

• Length: 264 bp

>T20555 NC_002945:3138750-3139013 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10183.40 Da
• Isoelectric Point: 4.3440

>AT20555 WP_003414599.1 NC_002945:3138465-3138746 [Mycobacterium tuberculosis variant bovis AF2122/97]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Nucleotide

• Length: 282 bp

>AT20555 NC_002945:3138465-3138746 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Structures

Toxin

• PDB: 3G5O

Antitoxin

• AlphaFold DB: A0A7U4BWP8