Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 26742..27264 Replicon plasmid pAVS0096-b
Accession NZ_CP076346
Organism Escherichia coli strain AVS0096

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag KM937_RS24600 Protein ID WP_000220561.1
Coordinates 26742..27023 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag KM937_RS24605 Protein ID WP_000121743.1
Coordinates 27013..27264 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KM937_RS24550 (22720) 22720..23265 - 546 WP_012301143.1 DNA distortion polypeptide 1 -
KM937_RS24560 (23596) 23596..23868 - 273 WP_000167280.1 hypothetical protein -
KM937_RS24565 (23885) 23885..24031 - 147 WP_001567307.1 hypothetical protein -
KM937_RS24570 (24101) 24101..24280 - 180 WP_000439079.1 hypothetical protein -
KM937_RS24575 (24322) 24322..24651 - 330 WP_085448582.1 hypothetical protein -
KM937_RS24580 (24745) 24745..24987 - 243 WP_000008708.1 hypothetical protein -
KM937_RS24585 (24977) 24977..25246 - 270 WP_001006718.1 hypothetical protein -
KM937_RS24590 (25255) 25255..25803 - 549 WP_001178641.1 hypothetical protein -
KM937_RS24595 (25877) 25877..26392 - 516 WP_085448581.1 J domain-containing protein -
KM937_RS24600 (26742) 26742..27023 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KM937_RS24605 (27013) 27013..27264 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
KM937_RS24610 (28502) 28502..29341 + 840 WP_001352874.1 replication initiation protein -
KM937_RS24615 (29362) 29362..30204 + 843 WP_072649828.1 replication initiation protein -
KM937_RS24620 (30208) 30208..30654 + 447 WP_085448580.1 hypothetical protein -
KM937_RS24625 (30791) 30791..31144 + 354 WP_001675596.1 DNA distortion polypeptide 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..44199 44199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T205544 WP_000220561.1 NZ_CP076346:c27023-26742 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T205544 NZ_CP101726:c4901743-4901459 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT205544 WP_000121743.1 NZ_CP076346:c27264-27013 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT205544 NZ_CP101726:c4901975-4901733 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References