Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 88989..89632 Replicon plasmid pAVS0096-a
Accession NZ_CP076345
Organism Escherichia coli strain AVS0096

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag KM937_RS24370 Protein ID WP_001034046.1
Coordinates 89216..89632 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag KM937_RS24365 Protein ID WP_001261278.1
Coordinates 88989..89219 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KM937_RS24335 (KM937_24335) 84500..84805 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
KM937_RS24340 (KM937_24340) 84807..85025 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
KM937_RS24345 (KM937_24345) 85592..86104 + 513 WP_000151784.1 hypothetical protein -
KM937_RS24350 (KM937_24350) 86138..87271 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
KM937_RS24355 (KM937_24355) 87438..88211 - 774 WP_000905949.1 hypothetical protein -
KM937_RS24360 (KM937_24360) 88224..88724 - 501 WP_001773886.1 HEPN family nuclease -
KM937_RS24365 (KM937_24365) 88989..89219 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KM937_RS24370 (KM937_24370) 89216..89632 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
KM937_RS24375 (KM937_24375) 89677..93471 - 3795 WP_001144732.1 hypothetical protein -
KM937_RS24380 (KM937_24380) 93852..94082 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
KM937_RS24385 (KM937_24385) 94079..94495 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aadA5 / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27 senB 1..101018 101018


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T205542 WP_001034046.1 NZ_CP076345:89216-89632 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T205542 NZ_CP101726:4186579-4186797 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT205542 WP_001261278.1 NZ_CP076345:88989-89219 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT205542 NZ_CP101726:4186178-4186552 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References