Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3592870..3593453 Replicon chromosome
Accession NZ_CP076344
Organism Escherichia coli strain AVS0096

Toxin (Protein)


Gene name mazF Uniprot ID S1PVD8
Locus tag KM937_RS17435 Protein ID WP_000254749.1
Coordinates 3592870..3593205 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag KM937_RS17440 Protein ID WP_000581937.1
Coordinates 3593205..3593453 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KM937_RS17420 (3588756) 3588756..3590054 - 1299 WP_000036723.1 phosphopyruvate hydratase -
KM937_RS17425 (3590142) 3590142..3591779 - 1638 WP_001774030.1 CTP synthase (glutamine hydrolyzing) -
KM937_RS17430 (3592007) 3592007..3592798 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
KM937_RS17435 (3592870) 3592870..3593205 - 336 WP_000254749.1 endoribonuclease MazF Toxin
KM937_RS17440 (3593205) 3593205..3593453 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KM937_RS17445 (3593531) 3593531..3595765 - 2235 WP_000226797.1 GTP diphosphokinase -
KM937_RS17450 (3595813) 3595813..3597114 - 1302 WP_000046817.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12137.06 Da        Isoelectric Point: 8.7218

>T205535 WP_000254749.1 NZ_CP076344:c3593205-3592870 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T205535 NZ_CP101719:c4262914-4262780 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT205535 WP_000581937.1 NZ_CP076344:c3593453-3593205 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT205535 NZ_CP101719:c4263141-4262989 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PVD8

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References