Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4579076..4579694 Replicon chromosome
Accession NZ_CP076318
Organism Escherichia coli strain Survcare045

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KNN08_RS23540 Protein ID WP_001291435.1
Coordinates 4579076..4579294 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KNN08_RS23545 Protein ID WP_000344800.1
Coordinates 4579320..4579694 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNN08_RS23505 (4574365) 4574365..4574937 + 573 WP_000779831.1 YbaY family lipoprotein -
KNN08_RS23510 (4574968) 4574968..4575279 - 312 WP_000409911.1 MGMT family protein -
KNN08_RS23520 (4575658) 4575658..4576011 + 354 WP_000878140.1 DUF1428 domain-containing protein -
KNN08_RS23525 (4576053) 4576053..4577603 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KNN08_RS23530 (4577767) 4577767..4578237 - 471 WP_000136192.1 YlaC family protein -
KNN08_RS23535 (4578353) 4578353..4578904 - 552 WP_000102564.1 maltose O-acetyltransferase -
KNN08_RS23540 (4579076) 4579076..4579294 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KNN08_RS23545 (4579320) 4579320..4579694 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KNN08_RS23550 (4580240) 4580240..4583389 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KNN08_RS23555 (4583412) 4583412..4584605 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T205511 WP_001291435.1 NZ_CP076318:c4579294-4579076 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T205511 NZ_CP101705:346657-347184 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT205511 WP_000344800.1 NZ_CP076318:c4579694-4579320 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT205511 NZ_CP101705:346384-346650 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References