Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3760914..3761411 Replicon chromosome
Accession NZ_CP076318
Organism Escherichia coli strain Survcare045

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KNN08_RS19635 Protein ID WP_001173343.1
Coordinates 3761238..3761411 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KNN08_RS19630 Protein ID WP_000398619.1
Coordinates 3760914..3761210 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNN08_RS19610 (3757645) 3757645..3758364 - 720 WP_000611288.1 two-component system response regulator DcuR -
KNN08_RS19615 (3758361) 3758361..3759992 - 1632 WP_001216477.1 sensor histidine kinase -
KNN08_RS19620 (3760173) 3760173..3760403 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KNN08_RS19625 (3760415) 3760415..3760687 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
KNN08_RS19630 (3760914) 3760914..3761210 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KNN08_RS19635 (3761238) 3761238..3761411 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KNN08_RS19640 (3761530) 3761530..3763047 - 1518 WP_001295074.1 lysine--tRNA ligase -
KNN08_RS19645 (3763284) 3763284..3764741 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T205506 WP_001173343.1 NZ_CP076318:3761238-3761411 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T205506 NZ_CP101704:c2863848-2863597 [Xanthomonas oryzae pv. oryzae]
ATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGGGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT205506 WP_000398619.1 NZ_CP076318:3760914-3761210 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT205506 NZ_CP101704:c2864153-2863875 [Xanthomonas oryzae pv. oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCGAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References