Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2310132..2310786 Replicon chromosome
Accession NZ_CP076318
Organism Escherichia coli strain Survcare045

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KNN08_RS12675 Protein ID WP_000244777.1
Coordinates 2310132..2310539 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KNN08_RS12680 Protein ID WP_000354046.1
Coordinates 2310520..2310786 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNN08_RS12655 (2306089) 2306089..2307822 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
KNN08_RS12660 (2307828) 2307828..2308538 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KNN08_RS12665 (2308563) 2308563..2309459 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KNN08_RS12670 (2309571) 2309571..2310092 + 522 WP_001055874.1 flavodoxin FldB -
KNN08_RS12675 (2310132) 2310132..2310539 - 408 WP_000244777.1 protein YgfX Toxin
KNN08_RS12680 (2310520) 2310520..2310786 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KNN08_RS12685 (2311029) 2311029..2312009 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KNN08_RS12690 (2312205) 2312205..2312864 - 660 WP_000250274.1 hemolysin III family protein -
KNN08_RS12695 (2313028) 2313028..2313339 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KNN08_RS12700 (2313384) 2313384..2314817 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
KNN08_RS12705 (2314874) 2314874..2315617 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T205501 WP_000244777.1 NZ_CP076318:c2310539-2310132 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T205501 NZ_CP101703:c2864913-2864662 [Xanthomonas oryzae pv. oryzae]
ATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGGGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT205501 WP_000354046.1 NZ_CP076318:c2310786-2310520 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT205501 NZ_CP101703:c2865218-2864940 [Xanthomonas oryzae pv. oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCGAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References