Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 16274..16799 Replicon plasmid pS045-CMY-42
Accession NZ_CP076315
Organism Escherichia coli strain Survcare045

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag KNN08_RS00760 Protein ID WP_001159871.1
Coordinates 16274..16579 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag KNN08_RS00765 Protein ID WP_000813630.1
Coordinates 16581..16799 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNN08_RS00745 (12236) 12236..13402 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
KNN08_RS00750 (13990) 13990..14745 - 756 WP_000852146.1 replication initiation protein RepE -
KNN08_RS00755 (15467) 15467..16273 - 807 WP_000016970.1 site-specific integrase -
KNN08_RS00760 (16274) 16274..16579 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
KNN08_RS00765 (16581) 16581..16799 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KNN08_RS00770 (17359) 17359..17589 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
KNN08_RS00775 (17586) 17586..18002 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
KNN08_RS00780 (18077) 18077..19642 + 1566 WP_001128474.1 AAA family ATPase -
KNN08_RS00785 (19627) 19627..20649 + 1023 WP_000361402.1 helicase UvrD -
KNN08_RS00790 (20903) 20903..21589 - 687 Protein_26 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCMY-42 - 1..167285 167285


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T205475 WP_001159871.1 NZ_CP076315:c16579-16274 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T205475 NZ_CP101689:1408977-1409504 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGAAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGCTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT205475 WP_000813630.1 NZ_CP076315:c16799-16581 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT205475 NZ_CP101689:1408690-1408980 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACATACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCGGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References