20547

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2840470..2841101 Replicon chromosome
Accession NC_002945
Organism Mycobacterium bovis AF2122/97

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag BQ2027_RS13155 Protein ID WP_003413174.1
Coordinates 2840724..2841101 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag BQ2027_RS13150 Protein ID WP_003413167.1
Coordinates 2840470..2840727 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BQ2027_RS13115 (BQ2027_MB2570) 2835728..2836042 + 315 WP_009937839.1 hypothetical protein -
BQ2027_RS13120 (BQ2027_MB2571) 2836338..2837549 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
BQ2027_RS13125 (BQ2027_MB2572) 2837676..2838335 + 660 WP_003900846.1 LppA family lipoprotein -
BQ2027_RS13130 (BQ2027_MB2573) 2838332..2838991 + 660 WP_003900847.1 LppA family lipoprotein -
BQ2027_RS13135 (BQ2027_MB2574) 2838988..2839650 + 663 WP_003900848.1 LppA family lipoprotein -
BQ2027_RS13140 (BQ2027_MB2575) 2839647..2839925 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
BQ2027_RS13145 (BQ2027_MB2576) 2840018..2840431 + 414 WP_003413164.1 PIN domain nuclease -
BQ2027_RS13150 (BQ2027_MB2577) 2840470..2840727 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
BQ2027_RS13155 (BQ2027_MB2578) 2840724..2841101 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
BQ2027_RS13160 (BQ2027_MB2578A) 2841117..2841491 - 375 WP_003413177.1 hypothetical protein -
BQ2027_RS13165 (BQ2027_MB2579C) 2841591..2841986 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
BQ2027_RS13170 (BQ2027_MB2580C) 2841983..2842228 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
BQ2027_RS13175 (BQ2027_MB2581C) 2842639..2843058 - 420 WP_003413190.1 A24 family peptidase -
BQ2027_RS13180 (BQ2027_MB2582C) 2843070..2843878 - 809 Protein_2636 shikimate dehydrogenase -
BQ2027_RS13185 (BQ2027_MB2583C) 2843875..2845128 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
BQ2027_RS13190 (BQ2027_MB2584C) 2845121..2845633 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T20547 WP_003413174.1 NC_002945:2840724-2841101 [Mycobacterium tuberculosis variant bovis AF2122/97]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T20547 NC_002945:2840724-2841101 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT20547 WP_003413167.1 NC_002945:2840470-2840727 [Mycobacterium tuberculosis variant bovis AF2122/97]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT20547 NC_002945:2840470-2840727 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References