Detailed information of TA system TA205469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 7174..7775
• Replicon: plasmid unnamed2
• Accession: NZ_CP076307
• Organism: Escherichia coli strain Survcare253

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: KNN07_RS23830
• Protein ID: WP_199613373.1
• Coordinates: 7174..7554 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: KNN07_RS23835
• Protein ID: WP_001190712.1
• Coordinates: 7554..7775 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNN07_RS23795 (KNN07_23795)	2205..3233	+	1029	WP_199613370.1	tyrosine-type recombinase/integrase	-
KNN07_RS23800 (KNN07_23800)	3307..3651	+	345	WP_001191776.1	hypothetical protein	-
KNN07_RS23805 (KNN07_23805)	3648..4124	+	477	WP_063074803.1	hypothetical protein	-
KNN07_RS23810 (KNN07_23810)	4121..4615	+	495	WP_000640907.1	dUTP diphosphatase	-
KNN07_RS23815 (KNN07_23815)	4630..5280	+	651	WP_199613371.1	DUF2829 domain-containing protein	-
KNN07_RS23820 (KNN07_23820)	5287..6054	+	768	WP_199613372.1	hypothetical protein	-
KNN07_RS23825 (KNN07_23825)	6044..7138	+	1095	WP_048666474.1	hypothetical protein	-
KNN07_RS23830 (KNN07_23830)	7174..7554	-	381	WP_199613373.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KNN07_RS23835 (KNN07_23835)	7554..7775	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KNN07_RS23840 (KNN07_23840)	7848..8237	-	390	WP_000506726.1	S24 family peptidase	-
KNN07_RS23845 (KNN07_23845)	8361..8612	-	252	WP_001283837.1	DNA polymerase III subunit theta	-
KNN07_RS25115	8645..8995	+	351	WP_001436207.1	hypothetical protein	-
KNN07_RS23855 (KNN07_23855)	8980..9264	-	285	WP_001142396.1	hypothetical protein	-
KNN07_RS25120	9248..10165	-	918	WP_244565733.1	hypothetical protein	-
KNN07_RS25125	10532..10630	-	99	Protein_16	hypothetical protein	-
KNN07_RS25130	10853..10978	-	126	Protein_17	hypothetical protein	-
KNN07_RS23865 (KNN07_23865)	10989..11252	-	264	WP_000224218.1	hypothetical protein	-
KNN07_RS23870 (KNN07_23870)	11254..11445	-	192	WP_199613375.1	hypothetical protein	-
KNN07_RS23875 (KNN07_23875)	11442..11618	-	177	WP_001261535.1	hypothetical protein	-
KNN07_RS23880 (KNN07_23880)	11618..12331	-	714	WP_199613376.1	ead/Ea22-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..86764	86764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13650.54 Da
• Isoelectric Point: 6.2282

>T205469 WP_199613373.1 NZ_CP076307:c7554-7174 [Escherichia coli]
MRHISPEELVALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDDPVLVELAVGAATGEIPVSSVAKKLRELYGSNI

Nucleotide

• Length: 381 bp

>T205469 NZ_CP101667:42068-42217 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACTGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT205469 WP_001190712.1 NZ_CP076307:c7775-7554 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT205469 NZ_CP101667:c42024-41963 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6