Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4231826..4232626 Replicon chromosome
Accession NZ_CP076305
Organism Escherichia coli strain Survcare253

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag KNN07_RS20685 Protein ID WP_000342449.1
Coordinates 4231826..4232353 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag KNN07_RS20690 Protein ID WP_001277108.1
Coordinates 4232360..4232626 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNN07_RS20660 (4226901) 4226901..4227668 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KNN07_RS20665 (4227665) 4227665..4228942 - 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
KNN07_RS20670 (4228939) 4228939..4229865 - 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KNN07_RS20675 (4229913) 4229913..4231022 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KNN07_RS20680 (4231446) 4231446..4231829 + 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
KNN07_RS20685 (4231826) 4231826..4232353 - 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KNN07_RS20690 (4232360) 4232360..4232626 - 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KNN07_RS20695 (4232776) 4232776..4233879 - 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KNN07_RS20700 (4234151) 4234151..4235005 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
KNN07_RS20705 (4235250) 4235250..4236308 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
KNN07_RS20710 (4236301) 4236301..4236969 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T205463 WP_000342449.1 NZ_CP076305:c4232353-4231826 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T205463 NZ_CP101666:3114598-3114705 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT205463 WP_001277108.1 NZ_CP076305:c4232626-4232360 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT205463 NZ_CP101666:c3114550-3114484 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References