Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3668560..3669214 Replicon chromosome
Accession NZ_CP076305
Organism Escherichia coli strain Survcare253

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KNN07_RS17840 Protein ID WP_000244777.1
Coordinates 3668560..3668967 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KNN07_RS17845 Protein ID WP_000354046.1
Coordinates 3668948..3669214 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNN07_RS17820 (3664517) 3664517..3666250 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
KNN07_RS17825 (3666256) 3666256..3666966 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KNN07_RS17830 (3666991) 3666991..3667887 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KNN07_RS17835 (3667999) 3667999..3668520 + 522 WP_001055874.1 flavodoxin FldB -
KNN07_RS17840 (3668560) 3668560..3668967 - 408 WP_000244777.1 protein YgfX Toxin
KNN07_RS17845 (3668948) 3668948..3669214 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KNN07_RS17850 (3669457) 3669457..3670437 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KNN07_RS17855 (3670514) 3670514..3671173 - 660 WP_000250274.1 hemolysin III family protein -
KNN07_RS17860 (3671337) 3671337..3671648 - 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
KNN07_RS17865 (3671693) 3671693..3673126 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
KNN07_RS17870 (3673183) 3673183..3673926 - 744 Protein_3514 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T205460 WP_000244777.1 NZ_CP076305:c3668967-3668560 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T205460 NZ_CP101666:2467341-2467718 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACCGAACAGTAAATAACATTACTCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT205460 WP_000354046.1 NZ_CP076305:c3669214-3668948 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT205460 NZ_CP101666:2466883-2467251 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCACGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References