Detailed information of TA system TA205458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3230279..3230904
• Replicon: chromosome
• Accession: NZ_CP076305
• Organism: Escherichia coli strain Survcare253

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KNN07_RS15765
• Protein ID: WP_000911330.1
• Coordinates: 3230279..3230677 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: KNN07_RS15770
• Protein ID: WP_000450524.1
• Coordinates: 3230677..3230904 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNN07_RS15745 (3226157)	3226157..3226357	+	201	WP_000383836.1	YpfN family protein	-
KNN07_RS15750 (3226467)	3226467..3227165	-	699	WP_000679823.1	esterase	-
KNN07_RS15755 (3227239)	3227239..3229254	-	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
KNN07_RS15760 (3229269)	3229269..3230132	-	864	WP_001267507.1	neutral zinc metallopeptidase	-
KNN07_RS15765 (3230279)	3230279..3230677	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
KNN07_RS15770 (3230677)	3230677..3230904	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KNN07_RS15775 (3231058)	3231058..3231771	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
KNN07_RS15780 (3231984)	3231984..3233018	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
KNN07_RS15785 (3233035)	3233035..3233913	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
KNN07_RS15790 (3234059)	3234059..3234631	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
KNN07_RS15795 (3234631)	3234631..3235101	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T205458 WP_000911330.1 NZ_CP076305:c3230677-3230279 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T205458 NZ_CP101666:c2260595-2260308 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGTATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT205458 WP_000450524.1 NZ_CP076305:c3230904-3230677 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT205458 NZ_CP101666:c2260321-2260019 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33