Detailed information of TA system TA020545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2825475..2826115
• Replicon: chromosome
• Accession: NC_002945
• Organism: Mycobacterium bovis AF2122/97

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: BQ2027_RS13055
• Protein ID: WP_003412970.1
• Coordinates: 2825475..2825894 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: BQ2027_RS13060
• Protein ID: WP_003412975.1
• Coordinates: 2825891..2826115 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BQ2027_RS13025 (BQ2027_MB2554C)	2821060..2821782	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
BQ2027_RS13030 (BQ2027_MB2555)	2822299..2822526	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
BQ2027_RS13035 (BQ2027_MB2556)	2822523..2822924	+	402	WP_003412963.1	PIN domain-containing protein	-
BQ2027_RS13040 (BQ2027_MB2557C)	2822959..2823879	-	921	WP_010950734.1	restriction endonuclease	-
BQ2027_RS13045	2824220..2824465	-	246	WP_162146607.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
BQ2027_RS13050 (BQ2027_MB2558)	2824524..2825474	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
BQ2027_RS13055 (BQ2027_MB2559C)	2825475..2825894	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
BQ2027_RS13060 (BQ2027_MB2559AC)	2825891..2826115	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
BQ2027_RS13065 (BQ2027_MB2560C)	2826146..2828989	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
BQ2027_RS13070 (BQ2027_MB2561C)	2829061..2829462	-	402	WP_003412981.1	hypothetical protein	-
BQ2027_RS13075 (BQ2027_MB2562C)	2829462..2829932	-	471	WP_003412985.1	transcription antitermination factor NusB	-
BQ2027_RS13080 (BQ2027_MB2563C)	2829935..2830498	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T20545 WP_003412970.1 NC_002945:c2825894-2825475 [Mycobacterium tuberculosis variant bovis AF2122/97]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T20545 NC_002945:c2825894-2825475 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT20545 WP_003412975.1 NC_002945:c2826115-2825891 [Mycobacterium tuberculosis variant bovis AF2122/97]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT20545 NC_002945:c2826115-2825891 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09