Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1041989..1042607 Replicon chromosome
Accession NZ_CP076305
Organism Escherichia coli strain Survcare253

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KNN07_RS05010 Protein ID WP_001291435.1
Coordinates 1041989..1042207 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KNN07_RS05015 Protein ID WP_000344800.1
Coordinates 1042233..1042607 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNN07_RS04975 (1037278) 1037278..1037850 + 573 WP_000779831.1 YbaY family lipoprotein -
KNN07_RS04980 (1037881) 1037881..1038192 - 312 WP_000409911.1 MGMT family protein -
KNN07_RS04990 (1038571) 1038571..1038924 + 354 WP_000878140.1 DUF1428 domain-containing protein -
KNN07_RS04995 (1038966) 1038966..1040516 - 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KNN07_RS05000 (1040680) 1040680..1041150 - 471 WP_000136192.1 YlaC family protein -
KNN07_RS05005 (1041266) 1041266..1041817 - 552 WP_000102568.1 maltose O-acetyltransferase -
KNN07_RS05010 (1041989) 1041989..1042207 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KNN07_RS05015 (1042233) 1042233..1042607 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KNN07_RS05020 (1043153) 1043153..1046302 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KNN07_RS05025 (1046325) 1046325..1047518 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T205447 WP_001291435.1 NZ_CP076305:c1042207-1041989 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T205447 NZ_CP101661:466739-467089 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT205447 WP_000344800.1 NZ_CP076305:c1042607-1042233 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT205447 NZ_CP101661:466452-466733 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References