Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 79732..80333 Replicon plasmid p321-pO111
Accession NZ_CP076303
Organism Escherichia coli strain Survcare321

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag KNN06_RS25475 Protein ID WP_001216045.1
Coordinates 79953..80333 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag KNN06_RS25470 Protein ID WP_001190712.1
Coordinates 79732..79953 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNN06_RS25460 (KNN06_25460) 77370..77996 - 627 WP_199613773.1 restriction endonuclease subunit S -
KNN06_RS25465 (KNN06_25465) 77993..79549 - 1557 WP_016231378.1 type I restriction-modification system subunit M -
KNN06_RS25470 (KNN06_25470) 79732..79953 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KNN06_RS25475 (KNN06_25475) 79953..80333 + 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KNN06_RS25480 (KNN06_25480) 80338..80517 + 180 WP_000113019.1 hypothetical protein -
KNN06_RS25485 (KNN06_25485) 80545..81588 + 1044 WP_016231380.1 DUF968 domain-containing protein -
KNN06_RS25490 (KNN06_25490) 81677..82129 + 453 WP_016231381.1 hypothetical protein -
KNN06_RS25495 (KNN06_25495) 82215..83408 + 1194 WP_016231382.1 hypothetical protein -
KNN06_RS25500 (KNN06_25500) 83408..84892 + 1485 WP_199613775.1 terminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..96219 96219


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T205441 WP_001216045.1 NZ_CP076303:79953-80333 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T205441 NZ_CP101656:2589906-2590775 [Pseudomonas aeruginosa]
ATGAACATGGCGCAGACCCACACCCCCGTCCCGAACCTGCAACAGCGCTTTCCCGAGCGGCTGGTGCAACTGGCCGACGG
CGCCCAGCTGGCCATCCGCGAATGCGGCCAGGGCCCGGTGGTGGTGCTGTTGCACGGCATCGGTTCGGGCTCGGCGTCCT
GGCTGCATTGCGCCCAGCGCCTGGCCGCCGGCAATCGGGTGATCGCCTGGGACGCGCCGGGTTACGGCCTGTCCACGCCG
CTGCCGCCGGCCCGGCCCAAGGCCTGCGACTACGCCGCGTGCCTGGAGCTGCTGCTGGATGCGCTGGGCGTGGAGAGCTG
CCTGCTGGTGGGCCACTCCCTCGGCGCGCTGATGGCCACGGCCTACGCCGCCGGCATCGGCGCGGCGCGGGTGCGCCGCC
TGGTGCTGCTCAGCCCGGCGCGCGGCTACGGCGCCGCCGAGCTGCGCGACAGCGGCGCCCAGGTGCGCCGCCAGCGCCTG
GAGAACCTGGAGCGCTTCGGTATCGACGGCATGGCCAGCGAGCGCACCGCGCGCCTGCTCGGCCGCAACCCCAGCGAGGA
GGCCCTGGCCTGGGTGCGCTGGAACATGGCGCGGCTCAACCCCGAGGGCTACCGCCAGGCCGTCGAGCTGCTCTGCGGCG
ACGACCTGCTGGGCAACGGGCAGCCTGCCGCTCCTTGCGAGGTGCATTGCGGCGAGGACGACGGCATCACCACGCCCGAG
AGTTGCGGCGCCATCGCCCGCCAGCTCGGCGCGTCGTTCTCGTCGATTCCCGGGGCCGGCCACGCCAGCCCGATCGAACA
ACCCGAAGTGGTCGCAGGACGCATCGGCCACGCGCAACGCCTCTCTCTTGAAGGAACCGCCAATGGATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT205441 WP_001190712.1 NZ_CP076303:79732-79953 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT205441 NZ_CP101656:2590768-2591601 [Pseudomonas aeruginosa]
ATGGATAAGTCAGACGATTCGCAAGACAAGTACATCGTGCCTGGCCTCGAACGCGGCCTGCTGCTGCTCTGCGAGTTCAG
CCGGAAGGACCGTACCCTGACGGCGCCGGAGTTGGCGCGGCGCCTGAAGCTGCCGCGCTCGACCATCTTCCGCCTGCTGA
CCACCCTGGAGGCCATGGGCTTCGTCACCCGCAACGGCAACGAGTACCGCCTGGGCATGGCGGTGTTGCGCCTGGGCTTC
GAGTACCTGGCCTCGCTGGAGCTGACCGAACTCGGCCAGCCGCTGCTGAACCGCCTGTGCGACGAGATCCGCTACCCCTG
CAACCTGGTGGTGCGCGACGGCCGTTCGATCGTCTACGTGGCCAAGGTTTCGCCGTCCACCCCGCTGAGCAGCTCGGTGA
ACGTCGGGACCCGGTTGCCGGCCCACGCCACGGTGCTCGGCCGCATCCTGCTGCAGGACCTGAGCCTGGGCGAGCTGCGC
GAGCTGTACCCGGAGGAGCAGCTGGAGCAGTTCTCGCCGAACACCCCGCGCAGCGTGCTGGAACTCTTCGACATGGTCCA
GGGCGACCGCCAGCGTGGCTTCGTCCAGGGCGAGGGGTTCTTCGAGGCGAGCATCTCCACCGTCGCGGCGCCGGTACGTG
ATCACAGCGGTCGCGTCATCGCGGCGATGGGGGCGACCATCGCCGCCGGGCATATCGACCCGGAACGCATCGAGGGACTG
GTCAGCCGGGTGCGCAGCAGCGCCGACGAGCTGTCGTACCTGCTCGACTACCGCGCCGACGGCCAGGACAACGTCACCCC
GATCTTCCGGAGTCGCCCGCATGAAACCGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References