205434

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4594947..4595565 Replicon chromosome
Accession NZ_CP076301
Organism Escherichia coli strain Survcare321

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KNN06_RS22065 Protein ID WP_001291435.1
Coordinates 4594947..4595165 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KNN06_RS22070 Protein ID WP_000344800.1
Coordinates 4595191..4595565 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNN06_RS22030 (4590236) 4590236..4590808 + 573 WP_000779831.1 YbaY family lipoprotein -
KNN06_RS22035 (4590839) 4590839..4591150 - 312 WP_000409911.1 MGMT family protein -
KNN06_RS22045 (4591529) 4591529..4591882 + 354 WP_000878140.1 DUF1428 domain-containing protein -
KNN06_RS22050 (4591924) 4591924..4593474 - 1551 WP_001577872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KNN06_RS22055 (4593638) 4593638..4594108 - 471 WP_001490888.1 YlaC family protein -
KNN06_RS22060 (4594224) 4594224..4594775 - 552 WP_001522034.1 maltose O-acetyltransferase -
KNN06_RS22065 (4594947) 4594947..4595165 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KNN06_RS22070 (4595191) 4595191..4595565 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KNN06_RS22075 (4596111) 4596111..4599260 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
KNN06_RS22080 (4599283) 4599283..4600476 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T205434 WP_001291435.1 NZ_CP076301:c4595165-4594947 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T205434 NZ_CP101648:3775867-3776085 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT205434 WP_000344800.1 NZ_CP076301:c4595565-4595191 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT205434 NZ_CP101648:3775465-3775839 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References