Detailed information of TA system TA020543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 2387118..2387647
• Replicon: chromosome
• Accession: NC_002945
• Organism: Mycobacterium bovis AF2122/97

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G0TMR4
• Locus tag: BQ2027_RS11025
• Protein ID: WP_003411124.1
• Coordinates: 2387118..2387435 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P9WJ74
• Locus tag: BQ2027_RS11030
• Protein ID: WP_003411127.1
• Coordinates: 2387432..2387647 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BQ2027_RS10995 (BQ2027_MB2162)	2382139..2383215	+	1077	WP_003411110.1	hypothetical protein	-
BQ2027_RS11000	2383212..2383493	+	282	WP_003411112.1	DUF5703 family protein	-
BQ2027_RS11005 (BQ2027_MB2163)	2383529..2384602	+	1074	WP_003411116.1	quinone-dependent dihydroorotate dehydrogenase	-
BQ2027_RS11010 (BQ2027_MB2164C)	2384607..2385137	-	531	WP_003411119.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
BQ2027_RS11015 (BQ2027_MB2165C)	2385185..2386531	-	1347	WP_003411121.1	M20/M25/M40 family metallo-hydrolase	-
BQ2027_RS11025 (BQ2027_MB2166C)	2387118..2387435	-	318	WP_003411124.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BQ2027_RS11030 (BQ2027_MB2166A)	2387432..2387647	-	216	WP_003411127.1	antitoxin ParD2	Antitoxin
BQ2027_RS11035 (BQ2027_MB2167)	2387902..2388960	+	1059	WP_003411129.1	phosphoribosyltransferase family protein	-
BQ2027_RS11040 (BQ2027_MB2168C)	2389090..2389446	-	357	WP_003411130.1	hypothetical protein	-
BQ2027_RS11045 (BQ2027_MB2169C)	2389541..2390323	-	783	WP_003411131.1	cell wall synthesis protein Wag31	-
BQ2027_RS11050 (BQ2027_MB2170C)	2390591..2390881	-	291	WP_003900476.1	YggT family protein	-
BQ2027_RS11055 (BQ2027_MB2171C)	2391043..2391699	-	657	WP_003411133.1	cell division protein SepF	-
BQ2027_RS11060 (BQ2027_MB2172C)	2391765..2392541	-	777	WP_003411137.1	YggS family pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-92)

Antitoxin

• Conserved hypothetical protein CHP02574, addiction module (11-69)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12312.88 Da
• Isoelectric Point: 6.4831

>T20543 WP_003411124.1 NC_002945:c2387435-2387118 [Mycobacterium tuberculosis variant bovis AF2122/97]
MTRRLRVHNGVEDDLFEAFSYYADAAPDQIDRLYNLFVDAVTKRIPQAPNAFAPLFKHYRHIYLRPFRYYVAYRTTDEAI
DILAVRHGMENPNAVEAEISGRTFE

Nucleotide

• Length: 318 bp

>T20543 NC_002945:c2387435-2387118 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7880.70 Da
• Isoelectric Point: 4.4055

>AT20543 WP_003411127.1 NC_002945:c2387647-2387432 [Mycobacterium tuberculosis variant bovis AF2122/97]
VVVNRALLASVDALSRDEQIELVEHINGNLAEGMHISEANQALIEARANDTDDAHWSTIDDFDKRIRARLG

Nucleotide

• Length: 216 bp

>AT20543 NC_002945:c2387647-2387432 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Structures

Toxin

• AlphaFold DB: G0TMR4

Antitoxin

• AlphaFold DB: A0A7U4FAJ4