Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2257709..2258363 Replicon chromosome
Accession NZ_CP076301
Organism Escherichia coli strain Survcare321

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag KNN06_RS10945 Protein ID WP_000244781.1
Coordinates 2257709..2258116 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KNN06_RS10950 Protein ID WP_000354046.1
Coordinates 2258097..2258363 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KNN06_RS10925 (2253666) 2253666..2255399 - 1734 WP_021523238.1 single-stranded-DNA-specific exonuclease RecJ -
KNN06_RS10930 (2255405) 2255405..2256115 - 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KNN06_RS10935 (2256140) 2256140..2257036 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KNN06_RS10940 (2257148) 2257148..2257669 + 522 WP_001521233.1 flavodoxin FldB -
KNN06_RS10945 (2257709) 2257709..2258116 - 408 WP_000244781.1 protein YgfX Toxin
KNN06_RS10950 (2258097) 2258097..2258363 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KNN06_RS10955 (2258606) 2258606..2259586 + 981 WP_000886050.1 tRNA-modifying protein YgfZ -
KNN06_RS10960 (2259663) 2259663..2260322 - 660 WP_000250269.1 hemolysin III family protein -
KNN06_RS10965 (2260486) 2260486..2260797 - 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
KNN06_RS10970 (2260842) 2260842..2262275 + 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T205422 WP_000244781.1 NZ_CP076301:c2258116-2257709 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T205422 NZ_CP101647:3775847-3776065 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT205422 WP_000354046.1 NZ_CP076301:c2258363-2258097 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT205422 NZ_CP101647:3775445-3775819 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References