Detailed information of TA system TA205418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 781425..782063
• Replicon: chromosome
• Accession: NZ_CP076301
• Organism: Escherichia coli strain Survcare321

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: KNN06_RS03920
• Protein ID: WP_001306887.1
• Coordinates: 781887..782063 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KNN06_RS03915
• Protein ID: WP_001270286.1
• Coordinates: 781425..781841 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNN06_RS03895 (776577)	776577..777518	-	942	WP_001251315.1	ABC transporter permease	-
KNN06_RS03900 (777519)	777519..778532	-	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
KNN06_RS03905 (778550)	778550..779695	-	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
KNN06_RS03910 (779940)	779940..781346	-	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
KNN06_RS03915 (781425)	781425..781841	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KNN06_RS03920 (781887)	781887..782063	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KNN06_RS03925 (782285)	782285..782515	+	231	WP_000494241.1	YncJ family protein	-
KNN06_RS03930 (782607)	782607..784568	-	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KNN06_RS03935 (784641)	784641..785177	-	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
KNN06_RS03940 (785269)	785269..786441	+	1173	WP_001523225.1	BenE family transporter YdcO	-
KNN06_RS03945 (786549)	786549..787007	-	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	786549..787007	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T205418 WP_001306887.1 NZ_CP076301:c782063-781887 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T205418 NZ_CP101647:1226196-1226609 [Salmonella enterica subsp. enterica serovar Kentucky]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAAAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT205418 WP_001270286.1 NZ_CP076301:c781841-781425 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT205418 NZ_CP101647:1225949-1226215 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76