20541

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2309155..2309791 Replicon chromosome
Accession NC_002945
Organism Mycobacterium bovis AF2122/97

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag BQ2027_RS10645 Protein ID WP_003410654.1
Coordinates 2309381..2309791 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag BQ2027_RS10640 Protein ID WP_003410651.1
Coordinates 2309155..2309388 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BQ2027_RS20415 (BQ2027_MB2086) 2304612..2305004 + 393 WP_229303073.1 metal ABC transporter permease -
BQ2027_RS10630 (BQ2027_MB2087C) 2305005..2305409 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
BQ2027_RS10635 (BQ2027_MB2088C) 2305493..2309077 - 3585 WP_010950652.1 cobaltochelatase subunit CobN -
BQ2027_RS10640 (BQ2027_MB2089) 2309155..2309388 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
BQ2027_RS10645 (BQ2027_MB2089A) 2309381..2309791 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
BQ2027_RS10650 (BQ2027_MB2090) 2309775..2310866 + 1092 WP_003900454.1 precorrin-3B synthase -
BQ2027_RS10655 (BQ2027_MB2091) 2310876..2311502 + 627 WP_003410658.1 precorrin-8X methylmutase -
BQ2027_RS10660 (BQ2027_MB2092) 2311499..2313025 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
BQ2027_RS10665 (BQ2027_MB2093C) 2312971..2314194 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T20541 WP_003410654.1 NC_002945:2309381-2309791 [Mycobacterium tuberculosis variant bovis AF2122/97]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T20541 NC_002945:2309381-2309791 [Mycobacterium tuberculosis variant bovis AF2122/97]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT20541 WP_003410651.1 NC_002945:2309155-2309388 [Mycobacterium tuberculosis variant bovis AF2122/97]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT20541 NC_002945:2309155-2309388 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BV50

References