205406

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 3083915..3084404 Replicon chromosome
Accession NZ_CP076298
Organism Burkholderia pseudomallei strain VBP21822

Toxin (Protein)


Gene name brnT Uniprot ID -
Locus tag KM155_RS30725 Protein ID WP_038734759.1
Coordinates 3084183..3084404 (-) Length 74 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag KM155_RS30720 Protein ID WP_004525420.1
Coordinates 3083915..3084199 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KM155_RS30695 (KM155_30695) 3079004..3080266 - 1263 WP_004551329.1 cytochrome c peroxidase -
KM155_RS30700 (KM155_30700) 3080425..3081885 - 1461 WP_207864755.1 metallophosphoesterase -
KM155_RS30705 (KM155_30705) 3082151..3082267 + 117 Protein_2548 IS3 family transposase -
KM155_RS30710 (KM155_30710) 3082494..3082922 - 429 Protein_2549 IS110 family transposase -
KM155_RS30715 (KM155_30715) 3082885..3083268 - 384 Protein_2550 integrase core domain-containing protein -
KM155_RS30720 (KM155_30720) 3083915..3084199 - 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
KM155_RS30725 (KM155_30725) 3084183..3084404 - 222 WP_038734759.1 BrnT family toxin Toxin
KM155_RS30730 (KM155_30730) 3085024..3085299 + 276 WP_038716556.1 hypothetical protein -
KM155_RS30735 (KM155_30735) 3085309..3085440 + 132 WP_009923452.1 bacteriophage protein Gp48 -
KM155_RS30740 (KM155_30740) 3085578..3085820 + 243 WP_004545584.1 hypothetical protein -
KM155_RS30745 (KM155_30745) 3085817..3085975 + 159 WP_004549802.1 hypothetical protein -
KM155_RS30750 (KM155_30750) 3086023..3086241 + 219 WP_004552392.1 hypothetical protein -
KM155_RS30755 (KM155_30755) 3086472..3087053 - 582 WP_004542548.1 hypothetical protein -
KM155_RS30760 (KM155_30760) 3087309..3087458 + 150 WP_004542626.1 bacteriophage protein Gp44 -
KM155_RS30765 (KM155_30765) 3087455..3087874 + 420 WP_243435357.1 SPFH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - ddhA 3017494..3095946 78452


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(14-65)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 74 a.a.        Molecular weight: 8606.71 Da        Isoelectric Point: 8.4920

>T205406 WP_038734759.1 NZ_CP076298:c3084404-3084183 [Burkholderia pseudomallei]
MECRWRLQRNSTGQDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREAKSYVEQA

Download         Length: 222 bp

>T205406 NZ_CP101618:49937-50071 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT205406 WP_004525420.1 NZ_CP076298:c3084199-3083915 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT205406 NZ_CP101618:c49892-49832 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References