Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | BrnTA/BrnT_toxin-BrnA |
| Location | 1656764..1657301 | Replicon | chromosome |
| Accession | NZ_CP076298 | ||
| Organism | Burkholderia pseudomallei strain VBP21822 | ||
Toxin (Protein)
| Gene name | brnT | Uniprot ID | Q2T6G2 |
| Locus tag | KM155_RS24990 | Protein ID | WP_004549731.1 |
| Coordinates | 1656764..1657033 (+) | Length | 90 a.a. |
Antitoxin (Protein)
| Gene name | brnA | Uniprot ID | Q2T6G1 |
| Locus tag | KM155_RS24995 | Protein ID | WP_011401115.1 |
| Coordinates | 1657017..1657301 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KM155_RS24950 (KM155_24950) | 1652138..1652959 | + | 822 | WP_004526649.1 | hypothetical protein | - |
| KM155_RS24955 (KM155_24955) | 1652956..1653216 | + | 261 | WP_004526650.1 | helix-turn-helix domain-containing protein | - |
| KM155_RS24960 (KM155_24960) | 1653370..1654362 | + | 993 | WP_076809500.1 | helix-turn-helix domain-containing protein | - |
| KM155_RS24965 (KM155_24965) | 1654359..1654910 | + | 552 | WP_122884277.1 | DUF1064 domain-containing protein | - |
| KM155_RS24970 (KM155_24970) | 1654907..1655281 | + | 375 | WP_038731729.1 | hypothetical protein | - |
| KM155_RS24975 (KM155_24975) | 1655278..1655703 | + | 426 | WP_207865012.1 | DUF1364 family protein | - |
| KM155_RS24980 (KM155_24980) | 1656035..1656274 | + | 240 | WP_243435371.1 | hypothetical protein | - |
| KM155_RS24985 (KM155_24985) | 1656271..1656714 | + | 444 | WP_076888524.1 | hypothetical protein | - |
| KM155_RS24990 (KM155_24990) | 1656764..1657033 | + | 270 | WP_004549731.1 | BrnT family toxin | Toxin |
| KM155_RS24995 (KM155_24995) | 1657017..1657301 | + | 285 | WP_011401115.1 | BrnA antitoxin family protein | Antitoxin |
| KM155_RS25000 (KM155_25000) | 1657479..1657673 | + | 195 | WP_207865010.1 | DUF2158 domain-containing protein | - |
| KM155_RS25005 (KM155_25005) | 1657854..1658240 | - | 387 | WP_004526660.1 | helix-turn-helix transcriptional regulator | - |
| KM155_RS25010 (KM155_25010) | 1658237..1658494 | - | 258 | WP_004549735.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| KM155_RS25015 (KM155_25015) | 1658553..1658909 | + | 357 | WP_004548856.1 | HNH endonuclease | - |
| KM155_RS25020 (KM155_25020) | 1659057..1659542 | + | 486 | WP_207865008.1 | phage terminase small subunit P27 family | - |
| KM155_RS25025 (KM155_25025) | 1659552..1661264 | + | 1713 | WP_207865006.1 | terminase large subunit | - |
| KM155_RS25030 (KM155_25030) | 1661261..1661446 | + | 186 | WP_015967349.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 1634386..1690104 | 55718 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 90 a.a. Molecular weight: 10157.46 Da Isoelectric Point: 5.6831
>T205404 WP_004549731.1 NZ_CP076298:1656764-1657033 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA
Download Length: 270 bp
>T205404 NZ_CP101618:c21205-20789 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10669.09 Da Isoelectric Point: 5.8930
>AT205404 WP_011401115.1 NZ_CP076298:1657017-1657301 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLKEHQPA
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLKEHQPA
Download Length: 285 bp
>AT205404 NZ_CP101618:c21432-21202 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|