Detailed information of TA system TA205401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1223199..1224116
• Replicon: chromosome
• Accession: NZ_CP076291
• Organism: Bacillus velezensis strain US1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: KM843_RS06425
• Protein ID: WP_012117368.1
• Coordinates: 1223370..1224116 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: KM843_RS06420
• Protein ID: WP_003154807.1
• Coordinates: 1223199..1223369 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KM843_RS06375 (KM843_06375)	1218333..1218845	+	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-
KM843_RS06380 (KM843_06380)	1218958..1219191	+	234	WP_012117363.1	XtmA	-
KM843_RS20700	1219207..1220055	+	849	WP_012117364.1	hypothetical protein	-
KM843_RS06390 (KM843_06390)	1220068..1220439	+	372	WP_012117365.1	XkdW family protein	-
KM843_RS06395 (KM843_06395)	1220444..1220641	+	198	WP_012117366.1	XkdX family protein	-
KM843_RS06400 (KM843_06400)	1220698..1221459	+	762	WP_012117367.1	hypothetical protein	-
KM843_RS06405 (KM843_06405)	1221511..1221774	+	264	WP_003154815.1	hemolysin XhlA family protein	-
KM843_RS06410 (KM843_06410)	1221788..1222051	+	264	WP_003154813.1	phage holin	-
KM843_RS06415 (KM843_06415)	1222065..1222943	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
KM843_RS06420 (KM843_06420)	1223199..1223369	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
KM843_RS06425 (KM843_06425)	1223370..1224116	-	747	WP_012117368.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KM843_RS06430 (KM843_06430)	1224221..1225219	-	999	WP_007407255.1	inorganic phosphate transporter	-
KM843_RS06435 (KM843_06435)	1225232..1225849	-	618	WP_003154804.1	DUF47 domain-containing protein	-
KM843_RS06440 (KM843_06440)	1226135..1227451	-	1317	WP_007610842.1	amino acid permease	-
KM843_RS06445 (KM843_06445)	1227774..1228724	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1210855..1281403	70548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29119.61 Da
• Isoelectric Point: 4.7003

>T205401 WP_012117368.1 NZ_CP076291:c1224116-1223370 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAEWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQADKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T205401 NZ_CP101615:c4247429-4247256 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT205401 WP_003154807.1 NZ_CP076291:c1223369-1223199 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT205401 NZ_CP101615:c4247753-4247457 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: I2HQ14