Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 488496..489133 | Replicon | chromosome |
| Accession | NZ_CP076291 | ||
| Organism | Bacillus velezensis strain US1 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | KM843_RS02490 | Protein ID | WP_003156187.1 |
| Coordinates | 488783..489133 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | I2HMS5 |
| Locus tag | KM843_RS02485 | Protein ID | WP_003156188.1 |
| Coordinates | 488496..488777 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KM843_RS02465 (KM843_02465) | 484861..485460 | - | 600 | WP_003156193.1 | rhomboid family intramembrane serine protease | - |
| KM843_RS02470 (KM843_02470) | 485553..485918 | + | 366 | WP_012116869.1 | holo-ACP synthase | - |
| KM843_RS02475 (KM843_02475) | 486083..487090 | + | 1008 | WP_007410230.1 | outer membrane lipoprotein carrier protein LolA | - |
| KM843_RS02480 (KM843_02480) | 487207..488376 | + | 1170 | WP_012116870.1 | alanine racemase | - |
| KM843_RS02485 (KM843_02485) | 488496..488777 | + | 282 | WP_003156188.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| KM843_RS02490 (KM843_02490) | 488783..489133 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| KM843_RS02495 (KM843_02495) | 489251..490072 | + | 822 | WP_003156182.1 | STAS domain-containing protein | - |
| KM843_RS02500 (KM843_02500) | 490077..490442 | + | 366 | WP_003156180.1 | RsbT antagonist protein RsbS | - |
| KM843_RS02505 (KM843_02505) | 490445..490846 | + | 402 | WP_003156178.1 | anti-sigma regulatory factor | - |
| KM843_RS02510 (KM843_02510) | 490858..491865 | + | 1008 | WP_007410233.1 | PP2C family protein-serine/threonine phosphatase | - |
| KM843_RS02515 (KM843_02515) | 491929..492258 | + | 330 | WP_003156176.1 | anti-sigma factor antagonist RsbV | - |
| KM843_RS02520 (KM843_02520) | 492255..492737 | + | 483 | WP_007609591.1 | anti-sigma B factor RsbW | - |
| KM843_RS02525 (KM843_02525) | 492703..493491 | + | 789 | WP_003156171.1 | RNA polymerase sigma factor SigB | - |
| KM843_RS02530 (KM843_02530) | 493491..494093 | + | 603 | WP_012116871.1 | PP2C family serine/threonine-protein phosphatase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T205400 WP_003156187.1 NZ_CP076291:488783-489133 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T205400 NZ_CP101615:3515619-3515837 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10562.92 Da Isoelectric Point: 6.4706
>AT205400 WP_003156188.1 NZ_CP076291:488496-488777 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT205400 NZ_CP101615:3515219-3515593 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|