205394

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 562706..563343 Replicon chromosome
Accession NZ_CP076290
Organism Bacillus velezensis strain Q12

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KM776_RS02805 Protein ID WP_003156187.1
Coordinates 562993..563343 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag KM776_RS02800 Protein ID WP_003156188.1
Coordinates 562706..562987 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KM776_RS02780 (559071) 559071..559670 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
KM776_RS02785 (559763) 559763..560128 + 366 WP_003156192.1 holo-ACP synthase -
KM776_RS02790 (560293) 560293..561300 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
KM776_RS02795 (561417) 561417..562586 + 1170 WP_069013421.1 alanine racemase -
KM776_RS02800 (562706) 562706..562987 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KM776_RS02805 (562993) 562993..563343 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KM776_RS02810 (563461) 563461..564282 + 822 WP_003156182.1 STAS domain-containing protein -
KM776_RS02815 (564287) 564287..564652 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
KM776_RS02820 (564655) 564655..565056 + 402 WP_003156178.1 anti-sigma regulatory factor -
KM776_RS02825 (565068) 565068..566075 + 1008 WP_025853725.1 PP2C family protein-serine/threonine phosphatase -
KM776_RS02830 (566139) 566139..566468 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
KM776_RS02835 (566465) 566465..566947 + 483 WP_003156173.1 anti-sigma B factor RsbW -
KM776_RS02840 (566913) 566913..567701 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
KM776_RS02845 (567701) 567701..568303 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T205394 WP_003156187.1 NZ_CP076290:562993-563343 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T205394 NZ_CP101615:2021361-2021464 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT205394 WP_003156188.1 NZ_CP076290:562706-562987 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT205394 NZ_CP101615:c2021502-2021231 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References