Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4381318..4381815 Replicon chromosome
Accession NZ_CP076285
Organism Escherichia coli strain 41

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag A7A12_RS21125 Protein ID WP_001173343.1
Coordinates 4381642..4381815 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag A7A12_RS21120 Protein ID WP_000398619.1
Coordinates 4381318..4381614 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A12_RS21100 (4378049) 4378049..4378768 - 720 WP_000611288.1 two-component system response regulator DcuR -
A7A12_RS21105 (4378765) 4378765..4380396 - 1632 WP_001216461.1 sensor histidine kinase -
A7A12_RS21110 (4380577) 4380577..4380807 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
A7A12_RS21115 (4380819) 4380819..4381091 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
A7A12_RS21120 (4381318) 4381318..4381614 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
A7A12_RS21125 (4381642) 4381642..4381815 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
A7A12_RS21130 (4381934) 4381934..4383451 - 1518 WP_001295074.1 lysine--tRNA ligase -
A7A12_RS21135 (4383688) 4383688..4385145 - 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T205393 WP_001173343.1 NZ_CP076285:4381642-4381815 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T205393 NZ_CP101615:1847166-1847420 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT205393 WP_000398619.1 NZ_CP076285:4381318-4381614 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT205393 NZ_CP101615:1846918-1847169 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References