Detailed information of TA system TA020539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2241371..2241997
• Replicon: chromosome
• Accession: NC_002945
• Organism: Mycobacterium bovis AF2122/97

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: BQ2027_RS10360
• Protein ID: WP_003410075.1
• Coordinates: 2241599..2241997 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BQ2027_RS10355
• Protein ID: WP_019283586.1
• Coordinates: 2241371..2241598 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BQ2027_RS10345 (BQ2027_MB2030C)	2239410..2239754	-	345	WP_003410065.1	ferredoxin family protein	-
BQ2027_RS10350 (BQ2027_MB2031C)	2239943..2241112	-	1170	WP_003899126.1	ATP-binding protein	-
BQ2027_RS10355 (BQ2027_MB2032)	2241371..2241598	+	228	WP_019283586.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BQ2027_RS10360 (BQ2027_MB2033)	2241599..2241997	+	399	WP_003410075.1	PIN domain nuclease	Toxin
BQ2027_RS10365 (BQ2027_MB2034C)	2242180..2242611	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
BQ2027_RS10370 (BQ2027_MB2035)	2242712..2243146	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
BQ2027_RS10375	2243583..2243762	-	180	Protein_2077	hypothetical protein	-
BQ2027_RS10380 (BQ2027_MB2036)	2243850..2244470	+	621	WP_003410086.1	IS110 family transposase	-
BQ2027_RS10385 (BQ2027_MB2037)	2244424..2245014	+	591	WP_003899131.1	IS110 family transposase	-
BQ2027_RS10390 (BQ2027_MB2038C)	2245142..2246398	-	1257	WP_010950642.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T20539 WP_003410075.1 NC_002945:2241599-2241997 [Mycobacterium tuberculosis variant bovis AF2122/97]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T20539 NC_002945:2241599-2241997 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8347.14 Da
• Isoelectric Point: 4.0995

>AT20539 WP_019283586.1 NC_002945:2241371-2241598 [Mycobacterium tuberculosis variant bovis AF2122/97]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSDDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT20539 NC_002945:2241371-2241598 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCGACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG