Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3059353..3060007 Replicon chromosome
Accession NZ_CP076285
Organism Escherichia coli strain 41

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag A7A12_RS14785 Protein ID WP_000244772.1
Coordinates 3059353..3059760 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A7A12_RS14790 Protein ID WP_000354046.1
Coordinates 3059741..3060007 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A12_RS14765 (3055310) 3055310..3057043 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
A7A12_RS14770 (3057049) 3057049..3057759 - 711 WP_000715212.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A7A12_RS14775 (3057784) 3057784..3058680 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
A7A12_RS14780 (3058792) 3058792..3059313 + 522 WP_001055874.1 flavodoxin FldB -
A7A12_RS14785 (3059353) 3059353..3059760 - 408 WP_000244772.1 protein YgfX Toxin
A7A12_RS14790 (3059741) 3059741..3060007 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A7A12_RS14795 (3060250) 3060250..3061230 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
A7A12_RS14800 (3061307) 3061307..3061966 - 660 WP_000250274.1 hemolysin III family protein -
A7A12_RS14805 (3062130) 3062130..3062441 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
A7A12_RS14810 (3062486) 3062486..3063919 + 1434 WP_157914749.1 6-phospho-beta-glucosidase BglA -
A7A12_RS14815 (3063976) 3063976..3064719 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T205389 WP_000244772.1 NZ_CP076285:c3059760-3059353 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T205389 NZ_CP101615:1046462-1046797 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT205389 WP_000354046.1 NZ_CP076285:c3060007-3059741 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT205389 NZ_CP101615:1046214-1046462 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References