20538

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2217631..2218217 Replicon chromosome
Accession NC_002945
Organism Mycobacterium bovis AF2122/97

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag BQ2027_RS10255 Protein ID WP_003410010.1
Coordinates 2217631..2217975 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag BQ2027_RS10260 Protein ID WP_003410014.1
Coordinates 2217969..2218217 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BQ2027_RS10220 (BQ2027_MB2008) 2213337..2213936 + 600 WP_003409989.1 L-lysine exporter -
BQ2027_RS10225 (BQ2027_MB2009) 2214352..2214780 + 429 WP_003409992.1 cellulose-binding protein -
BQ2027_RS10230 (BQ2027_MB2010) 2215006..2215545 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
BQ2027_RS10235 (BQ2027_MB2011C) 2216065..2216625 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
BQ2027_RS10240 (BQ2027_MB2012C) 2216622..2216963 - 342 WP_003410003.1 DUF2384 domain-containing protein -
BQ2027_RS10245 2217049..2217306 + 258 WP_003410006.1 hypothetical protein -
BQ2027_RS10250 (BQ2027_MB2013C) 2217207..2217542 - 336 WP_003410009.1 dehydrogenase -
BQ2027_RS10255 (BQ2027_MB2014C) 2217631..2217975 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
BQ2027_RS10260 (BQ2027_MB2014A) 2217969..2218217 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
BQ2027_RS10265 (BQ2027_MB2015C) 2218317..2220632 - 2316 WP_003899120.1 cation transporter ATPase CptG -
BQ2027_RS10270 (BQ2027_MB2016C) 2220629..2220901 - 273 WP_003410017.1 DUF1490 family protein -
BQ2027_RS10275 (BQ2027_MB2017C) 2220954..2221310 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
BQ2027_RS10280 (BQ2027_MB2018) 2221467..2222234 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T20538 WP_003410010.1 NC_002945:c2217975-2217631 [Mycobacterium tuberculosis variant bovis AF2122/97]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T20538 NC_002945:c2217975-2217631 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT20538 WP_003410014.1 NC_002945:c2218217-2217969 [Mycobacterium tuberculosis variant bovis AF2122/97]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT20538 NC_002945:c2218217-2217969 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4FAW9

References