Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 487590..488208 Replicon chromosome
Accession NZ_CP076285
Organism Escherichia coli strain 41

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag A7A12_RS02305 Protein ID WP_001291435.1
Coordinates 487590..487808 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag A7A12_RS02310 Protein ID WP_000344800.1
Coordinates 487834..488208 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A12_RS02270 (482880) 482880..483452 + 573 WP_000779826.1 YbaY family lipoprotein -
A7A12_RS02275 (483483) 483483..483794 - 312 WP_000409911.1 MGMT family protein -
A7A12_RS02285 (484173) 484173..484526 + 354 WP_000878141.1 DUF1428 family protein -
A7A12_RS02290 (484568) 484568..486118 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
A7A12_RS02295 (486282) 486282..486752 - 471 WP_000136192.1 YlaC family protein -
A7A12_RS02300 (486868) 486868..487419 - 552 WP_000102564.1 maltose O-acetyltransferase -
A7A12_RS02305 (487590) 487590..487808 - 219 WP_001291435.1 HHA domain-containing protein Toxin
A7A12_RS02310 (487834) 487834..488208 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
A7A12_RS02315 (488754) 488754..491903 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
A7A12_RS02320 (491926) 491926..493119 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T205370 WP_001291435.1 NZ_CP076285:c487808-487590 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T205370 NZ_CP101595:c2837116-2836784 [Cronobacter dublinensis]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGCCATGAACAAAGCGGAAAGCGCCCGGTGCTTAT
CGTATCGAAAGCCTCTTTTAACCATGTCACCCGGCTCCCCATCGTGCTTCCTGTAACGAGCGGCGGTAACTTTGCCCGTA
CGGCGGGTTTTGCCGTGTCGCTGGATGGAGCAGGAACCAAAACCACCGGCGTGATCCGCTGCGACCAGCCGAGAACCATT
GATATGGAAGCGCGCCACGGCATACGGCTGGAACGGCTACCCGACCATGTCGTCAATGAGGTACTGGCGAGGCTTGAAGC
CATTTTGAACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT205370 WP_000344800.1 NZ_CP076285:c488208-487834 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT205370 NZ_CP101595:c2837374-2837117 [Cronobacter dublinensis]
ATGTTTACCACTCGTCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCTGCCGTGCTGAAAACGCTGGGGCT
GTCAACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGATGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGACGAAGAGCGGGAATGGATTGATACGCCTGCCGTG
GGCAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References