20537

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mbcAT/RES-TIGR02293
Location 2216065..2216963 Replicon chromosome
Accession NC_002945
Organism Mycobacterium bovis AF2122/97

Toxin (Protein)


Gene name mbcT Uniprot ID P64908
Locus tag BQ2027_RS10235 Protein ID WP_003410001.1
Coordinates 2216065..2216625 (-) Length 187 a.a.

Antitoxin (Protein)


Gene name mbcA Uniprot ID P64910
Locus tag BQ2027_RS10240 Protein ID WP_003410003.1
Coordinates 2216622..2216963 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BQ2027_RS10205 (BQ2027_MB2006C) 2211234..2211887 - 654 WP_003409976.1 cutinase Cfp21 -
BQ2027_RS10210 2212002..2212232 + 231 WP_003409980.1 DUF167 domain-containing protein -
BQ2027_RS10215 (BQ2027_MB2007C) 2212317..2213228 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
BQ2027_RS10220 (BQ2027_MB2008) 2213337..2213936 + 600 WP_003409989.1 L-lysine exporter -
BQ2027_RS10225 (BQ2027_MB2009) 2214352..2214780 + 429 WP_003409992.1 cellulose-binding protein -
BQ2027_RS10230 (BQ2027_MB2010) 2215006..2215545 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
BQ2027_RS10235 (BQ2027_MB2011C) 2216065..2216625 - 561 WP_003410001.1 RES family NAD+ phosphorylase Toxin
BQ2027_RS10240 (BQ2027_MB2012C) 2216622..2216963 - 342 WP_003410003.1 DUF2384 domain-containing protein Antitoxin
BQ2027_RS10245 2217049..2217306 + 258 WP_003410006.1 hypothetical protein -
BQ2027_RS10250 (BQ2027_MB2013C) 2217207..2217542 - 336 WP_003410009.1 dehydrogenase -
BQ2027_RS10255 (BQ2027_MB2014C) 2217631..2217975 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 -
BQ2027_RS10260 (BQ2027_MB2014A) 2217969..2218217 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family -
BQ2027_RS10265 (BQ2027_MB2015C) 2218317..2220632 - 2316 WP_003899120.1 cation transporter ATPase CptG -
BQ2027_RS10270 (BQ2027_MB2016C) 2220629..2220901 - 273 WP_003410017.1 DUF1490 family protein -
BQ2027_RS10275 (BQ2027_MB2017C) 2220954..2221310 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(25-148)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(69-110)


Sequences

Toxin        


Download         Length: 187 a.a.        Molecular weight: 20248.70 Da        Isoelectric Point: 4.5091

>T20537 WP_003410001.1 NC_002945:c2216625-2216065 [Mycobacterium tuberculosis variant bovis AF2122/97]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Download         Length: 561 bp

>T20537 NC_002945:c2216625-2216065 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGAGCGATGCCCTCGATGAAGGGCTCGTCCAGCGTATCGACGCACGCGGAACAATTGAGTGGTCGGAAACGTGCTACCG
GTATACCGGCGCGCACCGTGACGCCTTGTCCGGTGAGGGCGCGCGCAGATTCGGAGGCAGGTGGAATCCGCCGCTGCTCT
TTCCGGCGATCTATCTTGCTGATTCCGCCCAAGCCTGCATGGTTGAGGTGGAACGGGCGGCGCAAGCGGCTTCAACGACC
GCAGAGAAGATGCTCGAGGCGGCCTACCGACTACACACGATCGACGTCACGGACCTGGCCGTCCTCGATCTGACAACCCC
GCAAGCTCGGGAAGCCGTGGGGCTCGAGAACGACGACATCTATGGCGACGACTGGTCAGGGTGCCAGGCGGTCGGACATG
CGGCCTGGTTCTTGCACATGCAAGGTGTCCTCGTGCCGGCGGCGGGCGGTGTCGGCCTCGTTGTCACCGCGTATGAACAG
CGAACTCGGCCGGGCCAACTACAACTGCGACAAAGCGTCGATCTGACGCCTGCTCTTTACCAAGAACTTCGAGCCACGTA
G

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12488.33 Da        Isoelectric Point: 4.8359

>AT20537 WP_003410003.1 NC_002945:c2216963-2216622 [Mycobacterium tuberculosis variant bovis AF2122/97]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Download         Length: 342 bp

>AT20537 NC_002945:c2216963-2216622 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGGGAGTAAATGTGCTCGCCTCGACCGTGTCGGGTGCGATCGAGCGCTTGGGATTGACCTACGAGGAAGTCGGTGACAT
CGTCGATGCCTCGCCGCGTTCCGTGGCGCGATGGACCGCAGGTCAGGTGGTTCCCCAACGCCTCAACAAGCAACGACTTA
TCGAGCTGGCCTATGTCGCCGACGCCCTCGCGGAAGTGCTGCCGCGTGACCAGGCGAACGTGTGGATGTTTTCGCCGAAT
CGGTTACTGGAACACCGCAAGCCTGCCGACCTCGTGCGAGACGGCGAGTACCAACGCGTGTTGGCGCTCATCGACGCGAT
GGCGGAGGGAGTGTTCGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FKG

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4FAE4

References