Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 453295..454132 Replicon chromosome
Accession NZ_CP076285
Organism Escherichia coli strain 41

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag A7A12_RS02135 Protein ID WP_000227784.1
Coordinates 453295..453837 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag A7A12_RS02140 Protein ID WP_001297137.1
Coordinates 453821..454132 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A12_RS02115 (448834) 448834..449745 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
A7A12_RS02120 (449913) 449913..450404 + 492 WP_001138904.1 nucleotide binding protein YajQ -
A7A12_RS02125 (450532) 450532..451896 - 1365 WP_001000960.1 MFS transporter -
A7A12_RS02130 (452304) 452304..453239 + 936 WP_001368479.1 tetratricopeptide repeat protein -
A7A12_RS02135 (453295) 453295..453837 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
A7A12_RS02140 (453821) 453821..454132 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
A7A12_RS02145 (454317) 454317..455207 - 891 WP_000971336.1 heme o synthase -
A7A12_RS02150 (455219) 455219..455548 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
A7A12_RS02155 (455548) 455548..456162 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
A7A12_RS02160 (456152) 456152..458143 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
A7A12_RS02165 (458165) 458165..459112 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T205369 WP_000227784.1 NZ_CP076285:c453837-453295 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T205369 NZ_CP101595:c2682380-2682277 [Cronobacter dublinensis]
GGCAAGGCGACTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTATCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTTTTTTT

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT205369 WP_001297137.1 NZ_CP076285:c454132-453821 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT205369 NZ_CP101595:2682274-2682415 [Cronobacter dublinensis]
GATAAAAAAAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGATAGAGCCGTGCGCTAAAAGTTGG
CATTAATGCAGGCTCAGTCGCCTTGCCATTTAAGAATAGATGACGACAGCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References