205367

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/Xre(antitoxin)
Location 50333..50834 Replicon plasmid p4422IncI2
Accession NZ_CP076284
Organism Escherichia coli strain 442/2

Toxin (Protein)


Gene name TsxA Uniprot ID A0A2S5RIK4
Locus tag A7A15_RS24280 Protein ID WP_022645141.1
Coordinates 50574..50834 (-) Length 87 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID A0A2S5RIE4
Locus tag A7A15_RS24275 Protein ID WP_022645142.1
Coordinates 50333..50584 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A15_RS24235 (A7A15_24115) 45698..46297 + 600 WP_000091423.1 hypothetical protein -
A7A15_RS24240 46317..46436 + 120 Protein_50 hypothetical protein -
A7A15_RS24245 (A7A15_24125) 46771..46949 + 179 Protein_51 hypothetical protein -
A7A15_RS24250 (A7A15_24130) 47283..47603 + 321 WP_001310435.1 hypothetical protein -
A7A15_RS24255 (A7A15_24135) 47734..48171 - 438 WP_000466318.1 type II toxin-antitoxin system HicB family antitoxin -
A7A15_RS24260 (A7A15_24140) 48196..48378 - 183 WP_000833470.1 type II toxin-antitoxin system HicA family toxin -
A7A15_RS24265 48675..49118 + 444 WP_000192626.1 hypothetical protein -
A7A15_RS24270 (A7A15_24150) 49505..49783 - 279 WP_000545938.1 hypothetical protein -
A7A15_RS24275 (A7A15_24155) 50333..50584 - 252 WP_022645142.1 transcriptional regulator Antitoxin
A7A15_RS24280 (A7A15_24160) 50574..50834 - 261 WP_022645141.1 hypothetical protein Toxin
A7A15_RS24285 (A7A15_24165) 50886..51110 - 225 WP_000814383.1 hypothetical protein -
A7A15_RS24290 (A7A15_24170) 51208..51417 - 210 WP_000866037.1 hypothetical protein -
A7A15_RS24295 (A7A15_24175) 51458..51781 - 324 WP_000427402.1 hypothetical protein -
A7A15_RS24300 (A7A15_24180) 52360..52689 - 330 WP_000855121.1 hypothetical protein -
A7A15_RS24305 (A7A15_24185) 53286..53951 + 666 WP_000532148.1 division plane positioning ATPase MipZ -
A7A15_RS24310 (A7A15_24190) 54025..54309 + 285 WP_001162371.1 hypothetical protein -
A7A15_RS24315 (A7A15_24195) 54327..54923 - 597 WP_000125164.1 ProQ/FINO family protein -
A7A15_RS24320 (A7A15_24200) 55073..55192 - 120 WP_227525580.1 Hok/Gef family protein -
A7A15_RS24325 55140..55292 - 153 Protein_67 FlmC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..58159 58159


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(8-37)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 9876.23 Da        Isoelectric Point: 7.2030

>T205367 WP_022645141.1 NZ_CP076284:c50834-50574 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEEAFDTIDFASDGLRENDKEIVSMMIQEMS
EEKNAN

Download         Length: 261 bp

>T205367 NZ_CP101595:224191-224718 [Cronobacter dublinensis]
ATGACGCTTAAGCGAGGCGTTTTTTCTTACGCACCGGAACACGGCTGGAAGACGGCGTCGTTCGACTGCGGCTGTGACAC
ACTGAATGATTTTCTTCGCCAGCGCCTTGCAGTGCAGCAGCAGCGCAATGTACTGCGCGCGTATGTCGTGTTAACCAACG
AGCCGGAGCCTGCGGTCGCTGGCTACTACACGCTGTCGGGCGCCTCTTTTGAGAGGTCGCTGTTTGGCGAACGGAAGCGG
CAGATCCCTTATGATAGCGCGCCATGCGTGTTACTGGGCAGACTGGCGGTAGACAAAACACATGCGGGAAAGGGCATAGG
GTCGATGCTGGTGGCGGATGCCGCGAGACGCGTTTATGACGTCGCCCGTGCGGTAGGCGTCTATGCGATGTTTGTGGAAG
CGAAAGATGCGCACGCCGCGCAGTTTTATCAGCAGCTCGGATTCCTGCAAGCAGAAAGTACCGACGAGCGACAGCGCCTG
TTTTTTCCGATCAGAGCACTCGACGCGTTATTCACTACAACACCATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9407.81 Da        Isoelectric Point: 9.8120

>AT205367 WP_022645142.1 NZ_CP076284:c50584-50333 [Escherichia coli]
MLTENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Download         Length: 252 bp

>AT205367 NZ_CP101595:223913-224194 [Cronobacter dublinensis]
ATGCCTGCGCCGTCGAAAAAAGACCGTCTGGAGTGTCGTCTCTCCACGGAGGACAAAGCTGCTATTGAAGCGGCCGCCAG
CCTCTCAAACACCAGCGTCTCGCAATTTATTGTCGATGCCGCTCGCGCTCGCGCCCGAGTCTTGCTTGAAGAACATCACC
GTATCCGGTTAACGGAGCAGGGCTGGCATGCCGTTATGGCGGCGCTGGATAACCCACCCGCGCCAAACGAGAAACTCAAG
CGTGCGTTTGAAAGACTGGATAACGGTGAATTCTGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S5RIK4

Antitoxin

Source ID Structure
AlphaFold DB A0A2S5RIE4

References