Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 42359..43162 Replicon plasmid p4422IncF
Accession NZ_CP076283
Organism Escherichia coli strain 442/2

Toxin (Protein)


Gene name KacT Uniprot ID A0A0K3X5E3
Locus tag A7A15_RS23845 Protein ID WP_000348884.1
Coordinates 42359..42889 (-) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID G3CAI7
Locus tag A7A15_RS23850 Protein ID WP_001275013.1
Coordinates 42893..43162 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A15_RS23815 (37612) 37612..38565 + 954 WP_001068902.1 ferric citrate uptake sigma factor regulator FecR -
A7A15_RS23820 (38652) 38652..40976 + 2325 WP_021534628.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
A7A15_RS23825 (41021) 41021..41108 + 88 Protein_54 hypothetical protein -
A7A15_RS23830 (41179) 41179..41451 + 273 WP_000019163.1 hypothetical protein -
A7A15_RS23835 (41432) 41432..42082 + 651 Protein_56 transposase zinc-binding domain-containing protein -
A7A15_RS23840 (42141) 42141..42358 - 218 Protein_57 transposase -
A7A15_RS23845 (42359) 42359..42889 - 531 WP_000348884.1 GNAT family N-acetyltransferase Toxin
A7A15_RS23850 (42893) 42893..43162 - 270 WP_001275013.1 DUF1778 domain-containing protein Antitoxin
A7A15_RS23855 (44050) 44050..45039 + 990 WP_024201093.1 RepB family plasmid replication initiator protein -
A7A15_RS23860 (45322) 45322..46062 - 741 WP_021534629.1 tyrosine-type recombinase/integrase -
A7A15_RS23865 (46183) 46183..46317 - 135 Protein_62 serine/threonine protein kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..77995 77995
- flank IS/Tn - - 41432..41797 365


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20320.22 Da        Isoelectric Point: 6.7486

>T205364 WP_000348884.1 NZ_CP076283:c42889-42359 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEVLFEVNDE

Download         Length: 531 bp

>T205364 NZ_CP101591:c4210721-4210413 [Cronobacter dublinensis]
ATGCAATATCAAATCTATCGCAATACCAGCACCAATAAAAAATACCCGTATTTAATAGACGTACAGAGCGATTTGATTGA
TGTATTAACCACCCGTGTCGTGATCCCGCTGGTGCCGCGCTTTATGGCGCAAAAACCACTGCCGGAAAGAATGTGTCCGG
TTGTTGAAGTCGATGGCGATGAATACATCGTTATGACGCATGAAATGGCAAGCGTCGGCAAAAGTGTACTTGGCGAGCAC
GCCGCCAGCGCATTACCCTGGCGTCAGCATATTAAAAACGCCATCGATTTTGTGTTCGACGGGTTTTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10229.63 Da        Isoelectric Point: 6.4702

>AT205364 WP_001275013.1 NZ_CP076283:c43162-42893 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Download         Length: 270 bp

>AT205364 NZ_CP101591:c4210962-4210723 [Cronobacter dublinensis]
ATGAACGTAGTCGTAAAACCGCCAAAAAGGACCGTTAGCGTGACGATAGACGAGGCGTTATACCAGCGCGCCAGAGCGCA
GGGCATCAATTTATCCCAACGGCTGAGCCTGGTGCTTGAAGAGGATCTGCGCGCAATCGAACGTCAGCAATGGAAAGACG
AAAATCGCGAAGGGTTGCTTGAACTGAACCGGATAGCCGACGAGTGTGGCCATTTCGCGGACGAACACCGGAAGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K3X5E3

Antitoxin

Source ID Structure
AlphaFold DB G3CAI7

References