Detailed information of TA system TA205360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 4727271..4728564
• Replicon: chromosome
• Accession: NZ_CP076282
• Organism: Escherichia coli strain 442/2

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: A0A3Z8A2R8
• Locus tag: A7A15_RS23040
• Protein ID: WP_000342496.1
• Coordinates: 4727659..4728564 (+)
• Length: 302 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: E0J2C6
• Locus tag: A7A15_RS23035
• Protein ID: WP_000152741.1
• Coordinates: 4727271..4727666 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A15_RS23020 (4723305)	4723305..4724510	-	1206	WP_001292892.1	restriction endonuclease subunit S	-
A7A15_RS23025 (4724507)	4724507..4726063	-	1557	WP_024232412.1	type I restriction-modification system subunit M	-
A7A15_RS23030 (4726368)	4726368..4727201	+	834	WP_001192742.1	DUF4942 domain-containing protein	-
A7A15_RS23035 (4727271)	4727271..4727666	+	396	WP_000152741.1	DUF6088 family protein	Antitoxin
A7A15_RS23040 (4727659)	4727659..4728564	+	906	WP_000342496.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
A7A15_RS23045 (4728928)	4728928..4729071	-	144	Protein_4494	HNH endonuclease	-
A7A15_RS23050 (4729208)	4729208..4729858	+	651	WP_001037970.1	HNH endonuclease	-
A7A15_RS23055 (4729866)	4729866..4730846	-	981	WP_001366032.1	sialate O-acetylesterase	-
A7A15_RS23060 (4730911)	4730911..4732017	-	1107	WP_001044128.1	N-acetylneuraminate epimerase	-
A7A15_RS23065 (4732037)	4732037..4732753	-	717	WP_001295734.1	N-acetylneuraminic acid outer membrane channel NanC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE	4727271..4735885	8614

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-300)

Antitoxin

• Protein of unknown function DUF6088 (7-108)

Sequences

Toxin

Protein

• Length: 302 a.a.
• Molecular weight: 34012.91 Da
• Isoelectric Point: 4.7207

>T205360 WP_000342496.1 NZ_CP076282:4727659-4728564 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYEMRYQQFVSPMVYGERKITWDEA

Nucleotide

• Length: 906 bp

>T205360 NZ_CP101591:c2758652-2758320 [Cronobacter dublinensis]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGCCATGAACAAAGCGGAAAGCGCCCGGTGCTTAT
CGTATCGAAAGCCTCTTTTAACCATGTCACCCGGCTCCCCATCGTGCTTCCTGTAACGAGCGGCGGCAACTTTGCCCGTA
CGGCGGGTTTTGCCGTGTCGCTGGAGGGGGCAGGAACCAAAACCACCGGCGTGATCCGCTGCGACCAGCCTAGAACCATT
GATATGGAAGCGCGCCACGGCATACGGCTGGAACGGCTACCCGACCATGTCGTGAATGAGGTGCTGGCGAGGCTCGAAGC
CATTTTGAACTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14619.93 Da
• Isoelectric Point: 11.6915

>AT205360 WP_000152741.1 NZ_CP076282:4727271-4727666 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRELVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Nucleotide

• Length: 396 bp

>AT205360 NZ_CP101591:c2758910-2758653 [Cronobacter dublinensis]
ATGTTTACCACTCGTCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGGGCT
GTCAACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGATGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGACGAAGATCGGGAATGGATTGATACGCCTGCCGTG
GGCAAGGAGATCCTGTAA

Structures

Toxin

• AlphaFold DB: A0A3Z8A2R8

Antitoxin

• AlphaFold DB: A0A1L4ZXD4