Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4573441..4573938 Replicon chromosome
Accession NZ_CP076282
Organism Escherichia coli strain 442/2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag A7A15_RS22290 Protein ID WP_001173343.1
Coordinates 4573765..4573938 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag A7A15_RS22285 Protein ID WP_000398619.1
Coordinates 4573441..4573737 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A15_RS22265 (4570172) 4570172..4570891 - 720 WP_000611288.1 two-component system response regulator DcuR -
A7A15_RS22270 (4570888) 4570888..4572519 - 1632 WP_001216486.1 sensor histidine kinase -
A7A15_RS22275 (4572700) 4572700..4572930 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
A7A15_RS22280 (4572942) 4572942..4573214 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
A7A15_RS22285 (4573441) 4573441..4573737 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
A7A15_RS22290 (4573765) 4573765..4573938 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
A7A15_RS22295 (4574057) 4574057..4575574 - 1518 WP_001295074.1 lysine--tRNA ligase -
A7A15_RS22300 (4575811) 4575811..4577268 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T205358 WP_001173343.1 NZ_CP076282:4573765-4573938 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T205358 NZ_CP101591:c1153399-1153181 [Cronobacter dublinensis]
ATGACGACCAAACCATTAACCAAAACCGATTATTTAATGCGACTGCGACGCTGTCAGACAATTGATACGCTTGAGCGCGT
GATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCGGTATTTTATTCAGCAGCGGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTTTACGATAAAATCCCAACGTCCGTGTGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT205358 WP_000398619.1 NZ_CP076282:4573441-4573737 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT205358 NZ_CP101591:c1153800-1153426 [Cronobacter dublinensis]
ATGGACGAATACTCACCAAAACGGCATGATATGGCGCAGCTTAAATTTCTGTGTGAAAGCCTGTATCATGATTGCCTCGC
CACGCTCGGCGAAAACCAGCATGGCTGGGTTAACGACCCCACATCCGCCGTTAACCTGCAACTGAACGATCTGATTGAGC
ACATTGCCAGCTTCGCGCTTAATTATAAAATTAAGCATGAAGAGGATGCCGCTCTCATTGAACAATTAGATGAATATCTG
GACGATACCTTTATGCTTTTCAGCAATTACGGTATCAATGCGCAAGATTTGCAAAAATGGCGCAAGTCCGGAAACAGACT
TTTCCGGTGTTTCGTAAACGCCAGCCGGGAGAATCCTGTGAGCTTGTCTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References