Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3784098..3784729 Replicon chromosome
Accession NZ_CP076282
Organism Escherichia coli strain 442/2

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag A7A15_RS18585 Protein ID WP_001259388.1
Coordinates 3784454..3784729 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag A7A15_RS18580 Protein ID WP_000593555.1
Coordinates 3784098..3784457 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A15_RS18550 (3779210) 3779210..3779683 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
A7A15_RS18555 (3779680) 3779680..3779961 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
A7A15_RS18560 (3780038) 3780038..3781396 + 1359 WP_001298729.1 PTS galactitol transporter subunit IIC -
A7A15_RS18565 (3781389) 3781389..3782897 + 1509 WP_001298718.1 FGGY-family carbohydrate kinase -
A7A15_RS18570 (3782887) 3782887..3783156 + 270 WP_000933060.1 HPr family phosphocarrier protein -
A7A15_RS18575 (3783188) 3783188..3784048 + 861 WP_001131783.1 class II aldolase -
A7A15_RS18580 (3784098) 3784098..3784457 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
A7A15_RS18585 (3784454) 3784454..3784729 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
A7A15_RS18590 (3784802) 3784802..3785926 - 1125 WP_001216257.1 ABC transporter permease -
A7A15_RS18595 (3785926) 3785926..3788661 - 2736 WP_000149124.1 ribosome-associated ATPase/putative transporter RbbA -
A7A15_RS18600 (3788658) 3788658..3789725 - 1068 WP_000361467.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T205356 WP_001259388.1 NZ_CP076282:c3784729-3784454 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T205356 NZ_CP101571:1604-1870 [Klebsiella pneumoniae subsp. pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT205356 WP_000593555.1 NZ_CP076282:c3784457-3784098 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT205356 NZ_CP101571:1396-1620 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References