Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3186828..3187482 Replicon chromosome
Accession NZ_CP076282
Organism Escherichia coli strain 442/2

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag A7A15_RS15565 Protein ID WP_000244772.1
Coordinates 3186828..3187235 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A7A15_RS15570 Protein ID WP_000354046.1
Coordinates 3187216..3187482 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A15_RS15545 (3182785) 3182785..3184518 - 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -
A7A15_RS15550 (3184524) 3184524..3185234 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A7A15_RS15555 (3185259) 3185259..3186155 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
A7A15_RS15560 (3186267) 3186267..3186788 + 522 WP_001055874.1 flavodoxin FldB -
A7A15_RS15565 (3186828) 3186828..3187235 - 408 WP_000244772.1 protein YgfX Toxin
A7A15_RS15570 (3187216) 3187216..3187482 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A7A15_RS15575 (3187725) 3187725..3188705 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
A7A15_RS15580 (3188782) 3188782..3189441 - 660 WP_000250274.1 hemolysin III family protein -
A7A15_RS15585 (3189605) 3189605..3189916 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
A7A15_RS15590 (3189961) 3189961..3191394 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
A7A15_RS15595 (3191451) 3191451..3192194 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T205354 WP_000244772.1 NZ_CP076282:c3187235-3186828 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T205354 NZ_CP101569:c103336-103187 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT205354 WP_000354046.1 NZ_CP076282:c3187482-3187216 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT205354 NZ_CP101569:103381-103440 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References