Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2727248..2727873 Replicon chromosome
Accession NZ_CP076282
Organism Escherichia coli strain 442/2

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag A7A15_RS13300 Protein ID WP_000911329.1
Coordinates 2727248..2727646 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag A7A15_RS13305 Protein ID WP_000450524.1
Coordinates 2727646..2727873 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A15_RS13280 (2723126) 2723126..2723326 + 201 WP_000383836.1 YpfN family protein -
A7A15_RS13285 (2723436) 2723436..2724134 - 699 WP_000679823.1 esterase -
A7A15_RS13290 (2724208) 2724208..2726223 - 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
A7A15_RS13295 (2726238) 2726238..2727101 - 864 WP_001267498.1 neutral zinc metallopeptidase -
A7A15_RS13300 (2727248) 2727248..2727646 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
A7A15_RS13305 (2727646) 2727646..2727873 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
A7A15_RS13310 (2728028) 2728028..2728741 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
A7A15_RS13315 (2728954) 2728954..2729988 - 1035 WP_001440783.1 outer membrane protein assembly factor BamC -
A7A15_RS13320 (2730005) 2730005..2730883 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
A7A15_RS13325 (2731029) 2731029..2731601 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
A7A15_RS13330 (2731601) 2731601..2732071 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T205352 WP_000911329.1 NZ_CP076282:c2727646-2727248 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T205352 NZ_CP101568:c172149-171838 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCACATTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT205352 WP_000450524.1 NZ_CP076282:c2727873-2727646 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT205352 NZ_CP101568:c171841-171422 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References