Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 490196..490814 Replicon chromosome
Accession NZ_CP076282
Organism Escherichia coli strain 442/2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag A7A15_RS02245 Protein ID WP_001291435.1
Coordinates 490196..490414 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag A7A15_RS02250 Protein ID WP_000344800.1
Coordinates 490440..490814 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A15_RS02210 (485486) 485486..486058 + 573 WP_000779831.1 YbaY family lipoprotein -
A7A15_RS02215 (486089) 486089..486400 - 312 WP_000409911.1 MGMT family protein -
A7A15_RS02225 (486779) 486779..487132 + 354 WP_000878140.1 DUF1428 domain-containing protein -
A7A15_RS02230 (487174) 487174..488724 - 1551 WP_096320443.1 cyclic-guanylate-specific phosphodiesterase PdeB -
A7A15_RS02235 (488888) 488888..489358 - 471 WP_000136192.1 YlaC family protein -
A7A15_RS02240 (489474) 489474..490025 - 552 WP_000102564.1 maltose O-acetyltransferase -
A7A15_RS02245 (490196) 490196..490414 - 219 WP_001291435.1 HHA domain-containing protein Toxin
A7A15_RS02250 (490440) 490440..490814 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
A7A15_RS02255 (491360) 491360..494509 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
A7A15_RS02260 (494532) 494532..495725 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T205337 WP_001291435.1 NZ_CP076282:c490414-490196 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T205337 NZ_CP101564:c5163-4747 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT205337 WP_000344800.1 NZ_CP076282:c490814-490440 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT205337 NZ_CP101564:c5390-5160 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References