Detailed information of TA system TA205336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 455901..456738
• Replicon: chromosome
• Accession: NZ_CP076282
• Organism: Escherichia coli strain 442/2

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: A7A15_RS02075
• Protein ID: WP_000227784.1
• Coordinates: 455901..456443 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: A7A15_RS02080
• Protein ID: WP_001297137.1
• Coordinates: 456427..456738 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A15_RS02055 (451440)	451440..452351	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
A7A15_RS02060 (452519)	452519..453010	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
A7A15_RS02065 (453138)	453138..454502	-	1365	WP_001000955.1	MFS transporter	-
A7A15_RS02070 (454910)	454910..455845	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
A7A15_RS02075 (455901)	455901..456443	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
A7A15_RS02080 (456427)	456427..456738	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
A7A15_RS02085 (456923)	456923..457813	-	891	WP_000971336.1	heme o synthase	-
A7A15_RS02090 (457825)	457825..458154	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
A7A15_RS02095 (458154)	458154..458768	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
A7A15_RS02100 (458758)	458758..460749	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
A7A15_RS02105 (460771)	460771..461718	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T205336 WP_000227784.1 NZ_CP076282:c456443-455901 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T205336 NZ_CP101563:c5363647-5363264 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT205336 WP_001297137.1 NZ_CP076282:c456738-456427 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT205336 NZ_CP101563:c5363889-5363647 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3