Detailed information of TA system TA205329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 138537..139072
• Replicon: plasmid pSA53IncF
• Accession: NZ_CP076280
• Organism: Escherichia coli strain SA53-3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: A7A19_RS25535
• Protein ID: WP_257122926.1
• Coordinates: 138785..139072 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: A7A19_RS25530
• Protein ID: WP_001132895.1
• Coordinates: 138537..138788 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A19_RS25500 (133910)	133910..134635	-	726	WP_160521638.1	protein mcbE	-
A7A19_RS25505 (134645)	134645..135832	-	1188	WP_257122924.1	YcaO-like family protein	-
A7A19_RS25510 (135825)	135825..136688	-	864	WP_257122925.1	SagB/ThcOx family dehydrogenase	-
A7A19_RS25515 (136650)	136650..137863	+	1214	WP_162829241.1	IS3 family transposase	-
A7A19_RS25520 (137866)	137866..137955	-	90	Protein_136	phage tail protein	-
A7A19_RS25525 (138235)	138235..138414	-	180	Protein_137	IS3 family transposase	-
A7A19_RS25530 (138537)	138537..138788	+	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
A7A19_RS25535 (138785)	138785..139072	+	288	WP_257122926.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A7A19_RS25540 (139074)	139074..139485	+	412	Protein_140	DDE-type integrase/transposase/recombinase	-
A7A19_RS25545 (140130)	140130..141107	+	978	WP_000361612.1	RepB family plasmid replication initiator protein	-
A7A19_RS25550 (141392)	141392..142132	-	741	WP_257122927.1	site-specific integrase	-
A7A19_RS25555 (142253)	142253..142440	-	188	Protein_143	serine/threonine protein kinase	-
A7A19_RS25560 (142815)	142815..143717	-	903	WP_001312822.1	antimicrobial resistance protein Mig-14	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyD / hlyB / hlyA / hlyC / espP	1..149690	149690

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11228.27 Da
• Isoelectric Point: 10.4703

>T205329 WP_257122926.1 NZ_CP076280:138785-139072 [Escherichia coli]
MRYQIKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T205329 NZ_CP101563:877784-878191 [Klebsiella pneumoniae subsp. pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT205329 WP_001132895.1 NZ_CP076280:138537-138788 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT205329 NZ_CP101563:877537-877803 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6