Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3321908..3322562 Replicon chromosome
Accession NZ_CP076279
Organism Escherichia coli strain SA53-3

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag A7A19_RS16555 Protein ID WP_000244777.1
Coordinates 3321908..3322315 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A7A19_RS16560 Protein ID WP_000354046.1
Coordinates 3322296..3322562 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A19_RS16535 (3317865) 3317865..3319598 - 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -
A7A19_RS16540 (3319604) 3319604..3320314 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A7A19_RS16545 (3320339) 3320339..3321235 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
A7A19_RS16550 (3321347) 3321347..3321868 + 522 WP_001055874.1 flavodoxin FldB -
A7A19_RS16555 (3321908) 3321908..3322315 - 408 WP_000244777.1 protein YgfX Toxin
A7A19_RS16560 (3322296) 3322296..3322562 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A7A19_RS16565 (3322805) 3322805..3323785 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
A7A19_RS16570 (3323981) 3323981..3324640 - 660 WP_000250274.1 hemolysin III family protein -
A7A19_RS16575 (3324804) 3324804..3325115 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
A7A19_RS16580 (3325160) 3325160..3326593 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
A7A19_RS16585 (3326650) 3326650..3327393 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T205319 WP_000244777.1 NZ_CP076279:c3322315-3321908 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T205319 NZ_CP101554:c4962053-4961769 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCTGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT205319 WP_000354046.1 NZ_CP076279:c3322562-3322296 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT205319 NZ_CP101554:c4962285-4962043 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References