Detailed information of TA system TA205316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2820674..2821299
• Replicon: chromosome
• Accession: NZ_CP076279
• Organism: Escherichia coli strain SA53-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A7A19_RS14040
• Protein ID: WP_000911330.1
• Coordinates: 2820674..2821072 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: A7A19_RS14045
• Protein ID: WP_000450524.1
• Coordinates: 2821072..2821299 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A19_RS14020 (2816581)	2816581..2816781	+	201	WP_000383836.1	YpfN family protein	-
A7A19_RS14025 (2816862)	2816862..2817560	-	699	WP_000679812.1	esterase	-
A7A19_RS14030 (2817634)	2817634..2819649	-	2016	WP_000829342.1	tRNA cytosine(34) acetyltransferase TmcA	-
A7A19_RS14035 (2819664)	2819664..2820527	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
A7A19_RS14040 (2820674)	2820674..2821072	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
A7A19_RS14045 (2821072)	2821072..2821299	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
A7A19_RS14050 (2821453)	2821453..2822166	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
A7A19_RS14055 (2822379)	2822379..2823413	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
A7A19_RS14060 (2823430)	2823430..2824308	-	879	WP_001345767.1	4-hydroxy-tetrahydrodipicolinate synthase	-
A7A19_RS14065 (2824454)	2824454..2825026	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
A7A19_RS14070 (2825026)	2825026..2825496	+	471	WP_257122557.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T205316 WP_000911330.1 NZ_CP076279:c2821072-2820674 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T205316 NZ_CP101554:4216311-4216529 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT205316 WP_000450524.1 NZ_CP076279:c2821299-2821072 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT205316 NZ_CP101554:4215910-4216284 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33