Detailed information of TA system TA205306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1772509..1774097
• Replicon: chromosome
• Accession: NZ_CP076279
• Organism: Escherichia coli strain SA53-3

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9XU38
• Locus tag: A7A19_RS08785
• Protein ID: WP_001125455.1
• Coordinates: 1772509..1773831 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: A7A19_RS08790
• Protein ID: WP_001296726.1
• Coordinates: 1773831..1774097 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A19_RS08760 (1768454)	1768454..1769017	-	564	WP_001296758.1	type 1 fimbrial major subunit FimA	-
A7A19_RS08765 (1769577)	1769577..1770002	+	426	WP_257115045.1	transposase	-
A7A19_RS08770 (1769999)	1769999..1770349	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
A7A19_RS08775 (1770380)	1770380..1771993	+	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
A7A19_RS08780 (1771980)	1771980..1772197	-	218	Protein_1719	AraC family transcriptional regulator	-
A7A19_RS08785 (1772509)	1772509..1773831	-	1323	WP_001125455.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
A7A19_RS08790 (1773831)	1773831..1774097	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49321.93 Da
• Isoelectric Point: 8.8753

>T205306 WP_001125455.1 NZ_CP076279:c1773831-1772509 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T205306 NZ_CP101546:c5172782-5172399 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT205306 WP_001296726.1 NZ_CP076279:c1774097-1773831 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT205306 NZ_CP101546:c5173024-5172782 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: U9XU38