Detailed information of TA system TA020530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1755351..1755964
• Replicon: chromosome
• Accession: NC_002945
• Organism: Mycobacterium bovis AF2122/97

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: BQ2027_RS08105
• Protein ID: WP_003407786.1
• Coordinates: 1755560..1755964 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: BQ2027_RS08100
• Protein ID: WP_009935474.1
• Coordinates: 1755351..1755554 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BQ2027_RS08085 (BQ2027_MB1583)	1750615..1753518	+	2904	WP_003407779.1	MMPL family transporter	-
BQ2027_RS08090 (BQ2027_MB1584)	1753528..1753974	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
BQ2027_RS08095 (BQ2027_MB1585)	1754009..1755298	+	1290	WP_003407781.1	threonine ammonia-lyase	-
BQ2027_RS08100 (BQ2027_MB1586)	1755351..1755554	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BQ2027_RS08105 (BQ2027_MB1587)	1755560..1755964	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
BQ2027_RS08110 (BQ2027_MB1588C)	1755981..1757723	-	1743	WP_010950559.1	malto-oligosyltrehalose trehalohydrolase	-
BQ2027_RS08115	1757716..1759207	-	1492	Protein_1627	alpha-amylase family glycosyl hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T20530 WP_003407786.1 NC_002945:1755560-1755964 [Mycobacterium tuberculosis variant bovis AF2122/97]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T20530 NC_002945:1755560-1755964 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT20530 WP_009935474.1 NC_002945:1755351-1755554 [Mycobacterium tuberculosis variant bovis AF2122/97]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT20530 NC_002945:1755351-1755554 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46