Detailed information of TA system TA205298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 84158..84801
• Replicon: plasmid pSA31IncI1
• Accession: NZ_CP076278
• Organism: Escherichia coli strain SA31-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: A7A21_RS24990
• Protein ID: WP_257115067.1
• Coordinates: 84385..84801 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A627FA37
• Locus tag: A7A21_RS24985
• Protein ID: WP_001604445.1
• Coordinates: 84158..84388 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A21_RS24955 (79377)	79377..79793	-	417	WP_001278818.1	plasmid partitioning/stability family protein	-
A7A21_RS24960 (79786)	79786..80766	-	981	WP_000688514.1	plasmid segregation protein ParM	-
A7A21_RS24965 (81180)	81180..81488	-	309	WP_000030199.1	hypothetical protein	-
A7A21_RS24970 (81575)	81575..82219	-	645	WP_001144036.1	ParA family protein	-
A7A21_RS24975 (82399)	82399..83178	-	780	WP_001164201.1	site-specific integrase	-
A7A21_RS24980 (83180)	83180..83593	-	414	WP_000465043.1	hypothetical protein	-
A7A21_RS24985 (84158)	84158..84388	+	231	WP_001604445.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
A7A21_RS24990 (84385)	84385..84801	+	417	WP_257115067.1	type II toxin-antitoxin system VapC family toxin	Toxin
A7A21_RS24995 (84971)	84971..86371	-	1401	Protein_98	AAA family ATPase	-
A7A21_RS25000 (86403)	86403..87995	-	1593	WP_257115068.1	IS66 family transposase	-
A7A21_RS25005 (88026)	88026..88376	-	351	WP_257115069.1	IS66 family insertion sequence element accessory protein TnpB	-
A7A21_RS25010 (88373)	88373..88807	-	435	WP_188926466.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	basG	1..115220	115220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15179.58 Da
• Isoelectric Point: 6.7132

>T205298 WP_257115067.1 NZ_CP076278:84385-84801 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTKEFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T205298 NZ_CP101546:722215-722700 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8736.84 Da
• Isoelectric Point: 4.4692

>AT205298 WP_001604445.1 NZ_CP076278:84158-84388 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVISDEGRFNL

Nucleotide

• Length: 231 bp

>AT205298 NZ_CP101546:721925-722218 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A627FA37