205293

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 137927..138462 Replicon plasmid pSA31IncF
Accession NZ_CP076277
Organism Escherichia coli strain SA31-1

Toxin (Protein)


Gene name relE Uniprot ID Q60228
Locus tag A7A21_RS24465 Protein ID WP_001270417.1
Coordinates 138175..138462 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag A7A21_RS24460 Protein ID WP_001132895.1
Coordinates 137927..138178 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A21_RS24435 (133729) 133729..134223 + 495 Protein_144 IS21 family transposase -
A7A21_RS24440 (134873) 134873..135774 + 902 Protein_145 DUF4158 domain-containing protein -
A7A21_RS24445 (136191) 136191..136297 - 107 Protein_146 ATP-binding protein -
A7A21_RS24450 (136409) 136409..137560 - 1152 WP_001254932.1 IS30-like element IS30 family transposase -
A7A21_RS24455 (137625) 137625..137804 - 180 Protein_148 IS3 family transposase -
A7A21_RS24460 (137927) 137927..138178 + 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
A7A21_RS24465 (138175) 138175..138462 + 288 WP_001270417.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A7A21_RS24470 (138464) 138464..138875 + 412 Protein_151 DDE-type integrase/transposase/recombinase -
A7A21_RS24475 (139520) 139520..140497 + 978 WP_000361610.1 RepB family plasmid replication initiator protein -
A7A21_RS24480 (140782) 140782..141522 - 741 WP_034173276.1 tyrosine-type recombinase/integrase -
A7A21_RS24485 (141643) 141643..141774 - 132 Protein_154 serine/threonine protein kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..149410 149410


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11214.24 Da        Isoelectric Point: 10.4703

>T205293 WP_001270417.1 NZ_CP076277:138175-138462 [Escherichia coli]
MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T205293 NZ_CP101543:c123770-123354 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT205293 WP_001132895.1 NZ_CP076277:137927-138178 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT205293 NZ_CP101543:c123997-123767 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5T8BSB4

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References