Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 103570..104095 | Replicon | plasmid pSA31IncF |
| Accession | NZ_CP076277 | ||
| Organism | Escherichia coli strain SA31-1 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | S1PFV8 |
| Locus tag | A7A21_RS24295 | Protein ID | WP_001159871.1 |
| Coordinates | 103570..103875 (-) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | Q3ZU16 |
| Locus tag | A7A21_RS24300 | Protein ID | WP_000813639.1 |
| Coordinates | 103877..104095 (-) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| A7A21_RS24280 (99519) | 99519..100685 | - | 1167 | WP_000772446.1 | plasmid-partitioning protein SopA | - |
| A7A21_RS24285 (101273) | 101273..102028 | - | 756 | WP_000852143.1 | replication initiation protein RepE | - |
| A7A21_RS24290 (102761) | 102761..103569 | - | 809 | Protein_115 | site-specific integrase | - |
| A7A21_RS24295 (103570) | 103570..103875 | - | 306 | WP_001159871.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
| A7A21_RS24300 (103877) | 103877..104095 | - | 219 | WP_000813639.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
| A7A21_RS24305 (104665) | 104665..105753 | + | 1089 | WP_257115058.1 | transcriptional repressor PifC | - |
| A7A21_RS24310 (105755) | 105755..107185 | + | 1431 | WP_251003572.1 | P-loop NTPase fold protein | - |
| A7A21_RS24315 (107261) | 107261..107641 | + | 381 | WP_001171554.1 | transposase | - |
| A7A21_RS24320 (107638) | 107638..107985 | + | 348 | WP_000612591.1 | IS66 family insertion sequence element accessory protein TnpB | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..149410 | 149410 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11692.49 Da Isoelectric Point: 6.4674
>T205292 WP_001159871.1 NZ_CP076277:c103875-103570 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T205292 NZ_CP101543:54187-54336 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8372.30 Da Isoelectric Point: 4.5203
>AT205292 WP_000813639.1 NZ_CP076277:c104095-103877 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT205292 NZ_CP101543:c54142-54083 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CCE8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U9DIR5 |